Potential for performance enhancement of biological wastewater treatment by bacterial community shift and adaptation of ammonia-oxidizing bacteria: a case study on the nitritation of pig slurry

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OriginalspracheEnglisch
Seiten (von - bis)185–201
Seitenumfang17
FachzeitschriftWater Science & Technology
Jahrgang93
Ausgabenummer2
Frühes Online-Datum14 Jan. 2026
PublikationsstatusVeröffentlicht - 15 Jan. 2026

Abstract

The bacterial groups responsible for nitrogen elimination in biological wastewater treatment are diverse and ubiquitous. This diversity implies
the possibility for the enhancement of biotechnological processes by natural selection (adaptation and community shift) in the activated
sludge under conditions usually considered unfavourable. The 1-year operation of a partial nitritation reactor– seeded with sludge from a
municipal single-stage deammoni cation reactor and fed with liquid pig slurry (high NH4-N and salinity)– revealed a combined functional
and structural change in the activated sludge and the ammonia-oxidizing bacteria (AOB) community to cope with the process’s conditions.
The relative abundance of AOB within the living microbial biomass increased from 22 to 35%. Initially, the AOB community consisted of Nitro-
somonas europaea/eutropha and Nitrosomonas communis. Following adaptation, members of the Nitrosomonas marina lineage and
Nitrosococcus mobilis were also identi ed within the sludge. AOB maximum activity (expressed as autotrophic speci c oxygen uptake
rate, SOUR) increased from 66.3 to 139.2 mgO2/gVSS/h, while the FA concentration associated with half-maximum SOUR (IC50) rose from
21.7 to 25.4 mg/L NH3 and 62.0 to 73.7 mg/L NH3. Natural selection acting on the microorganisms in the nitritation reactor helped to form
a highly specialized and productive activated sludge.

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abstract = "The bacterial groups responsible for nitrogen elimination in biological wastewater treatment are diverse and ubiquitous. This diversity impliesthe possibility for the enhancement of biotechnological processes by natural selection (adaptation and community shift) in the activatedsludge under conditions usually considered unfavourable. The 1-year operation of a partial nitritation reactor– seeded with sludge from amunicipal single-stage deammoni cation reactor and fed with liquid pig slurry (high NH4-N and salinity)– revealed a combined functionaland structural change in the activated sludge and the ammonia-oxidizing bacteria (AOB) community to cope with the process{\textquoteright}s conditions.The relative abundance of AOB within the living microbial biomass increased from 22 to 35%. Initially, the AOB community consisted of Nitro-somonas europaea/eutropha and Nitrosomonas communis. Following adaptation, members of the Nitrosomonas marina lineage andNitrosococcus mobilis were also identi ed within the sludge. AOB maximum activity (expressed as autotrophic speci c oxygen uptakerate, SOUR) increased from 66.3 to 139.2 mgO2/gVSS/h, while the FA concentration associated with half-maximum SOUR (IC50) rose from21.7 to 25.4 mg/L NH3 and 62.0 to 73.7 mg/L NH3. Natural selection acting on the microorganisms in the nitritation reactor helped to forma highly specialized and productive activated sludge.",
keywords = "ammonia-oxidizing bacteria, bacterial community, free ammonia adaptation, high-rate nitritation, nitrogen elimination, pig slurry",
author = "Johannes Reiter and Maike Beier and Stephan K{\"o}ster",
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AU - Reiter, Johannes

AU - Beier, Maike

AU - Köster, Stephan

N1 - Publisher Copyright: © 2026 The Authors.

PY - 2026/1/15

Y1 - 2026/1/15

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KW - bacterial community

KW - free ammonia adaptation

KW - high-rate nitritation

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