TY - JOUR

T1 - Pilot-Scale Production of the Natural Colorant Laetiporic Acid, Its Stability and Potential Applications

AU - Bergmann, Pia

AU - Frank, Christina

AU - Reinhardt, Olena

AU - Takenberg, Meike

AU - Werner, Anett

AU - Berger, Ralf G.

AU - Ersoy, Franziska

AU - Zschätzsch, Marlen

N1 - Funding Information: This research was funded by the German Federal Ministry of Education and Research (BMBF), grant number 031B1079 and 031B1079A. The application for third-party funding was partially financed by the project scouts (Forschungspool) of TU Dresden. The publication of this article was funded by the Open Access Fund of Leibniz Universität Hannover.

PY - 2022/12

Y1 - 2022/12

N2 - Laetiporus sulphureus, a wood-decaying basidiomycete, produces yellow-orange pigments in fruiting bodies and, as was recently shown, in submerged cultivated mycelia. Out of four strains, the most potent laetiporic acid producer was identified and its yield compared in different media. The complex Moser b medium was replaced by potato dextrose broth, achieving higher yields at a lower cost. Cultivation was then scaled up from shake flask to a 7 L stirred tank bioreactor. Optimization of parameters led to increased product concentrations up to 1 g L−1, the highest yield reported so far. An in situ product recovery strategy with a biphasic system was established, increasing the yield by 19% on the shake flask scale. A crude ethanolic extract of the biomass was examined for color stability and application trials. In contrast to what has been suggested in the past, the pigment showed limited long-term stability to oxygen and light, but was stable under storage in the dark at 4 °C under nitrogen. The orange extract was successfully incorporated into different matrices like foods, cosmetics and textiles. Laetiporic acid can potentially replace petrochemical based synthetic dyes, and can thus support the development of a circular bioeconomy.

AB - Laetiporus sulphureus, a wood-decaying basidiomycete, produces yellow-orange pigments in fruiting bodies and, as was recently shown, in submerged cultivated mycelia. Out of four strains, the most potent laetiporic acid producer was identified and its yield compared in different media. The complex Moser b medium was replaced by potato dextrose broth, achieving higher yields at a lower cost. Cultivation was then scaled up from shake flask to a 7 L stirred tank bioreactor. Optimization of parameters led to increased product concentrations up to 1 g L−1, the highest yield reported so far. An in situ product recovery strategy with a biphasic system was established, increasing the yield by 19% on the shake flask scale. A crude ethanolic extract of the biomass was examined for color stability and application trials. In contrast to what has been suggested in the past, the pigment showed limited long-term stability to oxygen and light, but was stable under storage in the dark at 4 °C under nitrogen. The orange extract was successfully incorporated into different matrices like foods, cosmetics and textiles. Laetiporic acid can potentially replace petrochemical based synthetic dyes, and can thus support the development of a circular bioeconomy.

KW - basidiomycete

KW - bioprocess

KW - biotechnology

KW - laetiporic acid

KW - Laetiporus sulphureus

KW - natural colorants

KW - natural dye

KW - submerged fermentation

UR - http://www.scopus.com/inward/record.url?scp=85144730419&partnerID=8YFLogxK

U2 - 10.3390/fermentation8120684

DO - 10.3390/fermentation8120684

M3 - Article

AN - SCOPUS:85144730419

VL - 8

JO - Fermentation

JF - Fermentation

IS - 12

M1 - 684

ER -