Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 331-334 |
| Seitenumfang | 4 |
| Fachzeitschrift | Biotechnology and Bioprocess Engineering |
| Jahrgang | 7 |
| Ausgabenummer | 6 |
| Publikationsstatus | Veröffentlicht - 2002 |
Abstract
Culture conductivity and on-line NADH fluorescence were used to measure cellular growth in plant cell suspension cultures of Podophyllum hexandrum. An inverse correlation between dry cell weight and medium conductivity was observed during shake flask cultivation. A linear relationship between dry cell weight and culture NADH fluorescence was obtained during the exponential phase of batch cultivation in a bioreactor under the pH stat (pH 6) conditions. It was observed that conductivity measurement were suitable for biomass characterisation under highly dynamic uncontrolled shake flask cultivation conditions. However, if the acid/alkali feeding is done for pH control the conductivity measurement could not be applied. On the other hand the NADH fluorescence measurement allowed online-in situ biomass monitoring of rather heteroge-nous plant cell suspension cultures in bioreactor even under the most desirable pH stat conditions.
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biotechnologie
- Chemische Verfahrenstechnik (insg.)
- Bioengineering
- Immunologie und Mikrobiologie (insg.)
- Angewandte Mikrobiologie und Biotechnologie
- Ingenieurwesen (insg.)
- Biomedizintechnik
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in: Biotechnology and Bioprocess Engineering, Jahrgang 7, Nr. 6, 2002, S. 331-334.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Non-invasive Methods for Determination of Cellular Growth in Podophyllum hexandrum Suspension Cultures
AU - Chattopadhyay, Saurabh
AU - Bisaria, V. S.
AU - Scheper, T.
AU - Srivastava, A. K.
PY - 2002
Y1 - 2002
N2 - Culture conductivity and on-line NADH fluorescence were used to measure cellular growth in plant cell suspension cultures of Podophyllum hexandrum. An inverse correlation between dry cell weight and medium conductivity was observed during shake flask cultivation. A linear relationship between dry cell weight and culture NADH fluorescence was obtained during the exponential phase of batch cultivation in a bioreactor under the pH stat (pH 6) conditions. It was observed that conductivity measurement were suitable for biomass characterisation under highly dynamic uncontrolled shake flask cultivation conditions. However, if the acid/alkali feeding is done for pH control the conductivity measurement could not be applied. On the other hand the NADH fluorescence measurement allowed online-in situ biomass monitoring of rather heteroge-nous plant cell suspension cultures in bioreactor even under the most desirable pH stat conditions.
AB - Culture conductivity and on-line NADH fluorescence were used to measure cellular growth in plant cell suspension cultures of Podophyllum hexandrum. An inverse correlation between dry cell weight and medium conductivity was observed during shake flask cultivation. A linear relationship between dry cell weight and culture NADH fluorescence was obtained during the exponential phase of batch cultivation in a bioreactor under the pH stat (pH 6) conditions. It was observed that conductivity measurement were suitable for biomass characterisation under highly dynamic uncontrolled shake flask cultivation conditions. However, if the acid/alkali feeding is done for pH control the conductivity measurement could not be applied. On the other hand the NADH fluorescence measurement allowed online-in situ biomass monitoring of rather heteroge-nous plant cell suspension cultures in bioreactor even under the most desirable pH stat conditions.
KW - Conductivity measurement
KW - NADH measurement
KW - On-line biomass measurement
KW - Podophyllum hexandrum
UR - http://www.scopus.com/inward/record.url?scp=50449101185&partnerID=8YFLogxK
U2 - 10.1007/BF02933516
DO - 10.1007/BF02933516
M3 - Article
AN - SCOPUS:50449101185
VL - 7
SP - 331
EP - 334
JO - Biotechnology and Bioprocess Engineering
JF - Biotechnology and Bioprocess Engineering
SN - 1226-8372
IS - 6
ER -