Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 7954-7960 |
| Seitenumfang | 7 |
| Fachzeitschrift | Analytical chemistry |
| Jahrgang | 97 |
| Ausgabenummer | 14 |
| Frühes Online-Datum | 5 Apr. 2025 |
| Publikationsstatus | Veröffentlicht - 15 Apr. 2025 |
Abstract
This study presents the first coupling of miniaturized chip-based supercritical fluid chromatography (SFC) with ion mobility spectrometry (IMS) enabling rapid two-dimensional analysis of moderately polar compounds. For the first time, ionization and analyte transfer at the SFC-IMS interface are achieved solely through eluent decompression in conjunction with a shifted electric IMS inlet potential. This straightforward approach significantly reduces instrumentation complexity and size, promoting system compactness and robustness. The integration of chip-based SFC with IMS enables high-speed separations of complex samples, drastically reducing analysis time while utilizing a detector capable of delivering structural information at a rapid acquisition rate and low cost. Evaluation of the SFC-IMS system as demonstrated through the chiral separation of Tröger’s base revealed exceptional repeatability and sensitivity. Short columns and high flow rates resulted in record-speed SFC-IMS analysis in just six seconds. The system was successfully used to analyze a complex mixture containing five isomers, including naloxone and 6-monoacetylmorphine, in just 30 s.
ASJC Scopus Sachgebiete
- Chemie (insg.)
- Analytische Chemie
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in: Analytical chemistry, Jahrgang 97, Nr. 14, 15.04.2025, S. 7954-7960.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Miniaturized Supercritical Fluid Chromatography Coupled with Ion Mobility Spectrometry
T2 - A Chip-Based Platform for Rapid Chiral and Complex Mixture Analysis
AU - Schwieger, Julius
AU - Welters, Klaus
AU - Thoben, Christian
AU - Nitschke, Alexander
AU - Zimmermann, Stefan
AU - Belder, Detlev
N1 - Publisher Copyright: © 2025 The Authors. Published by American Chemical Society.
PY - 2025/4/15
Y1 - 2025/4/15
N2 - This study presents the first coupling of miniaturized chip-based supercritical fluid chromatography (SFC) with ion mobility spectrometry (IMS) enabling rapid two-dimensional analysis of moderately polar compounds. For the first time, ionization and analyte transfer at the SFC-IMS interface are achieved solely through eluent decompression in conjunction with a shifted electric IMS inlet potential. This straightforward approach significantly reduces instrumentation complexity and size, promoting system compactness and robustness. The integration of chip-based SFC with IMS enables high-speed separations of complex samples, drastically reducing analysis time while utilizing a detector capable of delivering structural information at a rapid acquisition rate and low cost. Evaluation of the SFC-IMS system as demonstrated through the chiral separation of Tröger’s base revealed exceptional repeatability and sensitivity. Short columns and high flow rates resulted in record-speed SFC-IMS analysis in just six seconds. The system was successfully used to analyze a complex mixture containing five isomers, including naloxone and 6-monoacetylmorphine, in just 30 s.
AB - This study presents the first coupling of miniaturized chip-based supercritical fluid chromatography (SFC) with ion mobility spectrometry (IMS) enabling rapid two-dimensional analysis of moderately polar compounds. For the first time, ionization and analyte transfer at the SFC-IMS interface are achieved solely through eluent decompression in conjunction with a shifted electric IMS inlet potential. This straightforward approach significantly reduces instrumentation complexity and size, promoting system compactness and robustness. The integration of chip-based SFC with IMS enables high-speed separations of complex samples, drastically reducing analysis time while utilizing a detector capable of delivering structural information at a rapid acquisition rate and low cost. Evaluation of the SFC-IMS system as demonstrated through the chiral separation of Tröger’s base revealed exceptional repeatability and sensitivity. Short columns and high flow rates resulted in record-speed SFC-IMS analysis in just six seconds. The system was successfully used to analyze a complex mixture containing five isomers, including naloxone and 6-monoacetylmorphine, in just 30 s.
UR - http://www.scopus.com/inward/record.url?scp=105002035990&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.5c00227
DO - 10.1021/acs.analchem.5c00227
M3 - Article
VL - 97
SP - 7954
EP - 7960
JO - Analytical chemistry
JF - Analytical chemistry
SN - 0003-2700
IS - 14
ER -