Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 578-589 |
| Seitenumfang | 12 |
| Fachzeitschrift | Plant Breeding |
| Jahrgang | 136 |
| Ausgabenummer | 4 |
| Frühes Online-Datum | 26 Mai 2017 |
| Publikationsstatus | Veröffentlicht - Aug. 2017 |
Abstract
Cleome gynandra (L.) Briq. is an African leafy vegetable with a potential to improve food security and micronutrient deficiencies. Cytological traits, breeding biology and genetic diversity of 30 selected entries of C. gynandra from six African countries were investigated. The entries consisted of advanced lines, gene bank accessions and farmers’ cultivars. Our study revealed chromosome numbers of 2n = 34 in root tip metaphase cells from one entry. The 30 entries were found to be diploid with genome sizes ranging from 2.31 to 2.45 pg/2C. Hand pollination experiments were carried out to assess self-incompatibility within the entries and revealed that they are self- and cross-compatible. For genetic diversity studies within and among the entries, the pooled data of 499 polymorphic bands from 11 amplified fragment length polymorphism primer combinations and nine simple sequence repeat markers were used. The genetic distance among the entries ranged from 0.13 to 0.77. In a principal coordinate analysis, the farmers’ cultivars formed a cluster separate from the advanced lines and the gene bank entries, and the latter were not well resolved.
ASJC Scopus Sachgebiete
- Agrar- und Biowissenschaften (insg.)
- Agronomie und Nutzpflanzenwissenschaften
- Biochemie, Genetik und Molekularbiologie (insg.)
- Genetik
- Agrar- und Biowissenschaften (insg.)
- Pflanzenkunde
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in: Plant Breeding, Jahrgang 136, Nr. 4, 08.2017, S. 578-589.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Mating biology, nuclear DNA content and genetic diversity in spider plant (Cleome gynandra) germplasm from various African countries
AU - Omondi, Emmanuel O.
AU - Debener, Thomas
AU - Linde, Marcus
AU - Abukutsa-Onyango, Mary
AU - Dinssa, Fekadu F.
AU - Winkelmann, Traud
N1 - Funding Information: The authors are grateful for the funding within the project HORTINLEA by the German Federal Ministry of Education and Research (BMBF) and the German Federal Ministry of Economic Cooperation and Development (BMZ) within the framework of the GlobE-Global Food Security. The authors thank Diro Terefe for assisting in SSR marker designing, David Odongo for farmer's cultivar collection and WorldVeg for supplying plant material. Publisher Copyright: © 2017 Blackwell Verlag GmbH Copyright: Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2017/8
Y1 - 2017/8
N2 - Cleome gynandra (L.) Briq. is an African leafy vegetable with a potential to improve food security and micronutrient deficiencies. Cytological traits, breeding biology and genetic diversity of 30 selected entries of C. gynandra from six African countries were investigated. The entries consisted of advanced lines, gene bank accessions and farmers’ cultivars. Our study revealed chromosome numbers of 2n = 34 in root tip metaphase cells from one entry. The 30 entries were found to be diploid with genome sizes ranging from 2.31 to 2.45 pg/2C. Hand pollination experiments were carried out to assess self-incompatibility within the entries and revealed that they are self- and cross-compatible. For genetic diversity studies within and among the entries, the pooled data of 499 polymorphic bands from 11 amplified fragment length polymorphism primer combinations and nine simple sequence repeat markers were used. The genetic distance among the entries ranged from 0.13 to 0.77. In a principal coordinate analysis, the farmers’ cultivars formed a cluster separate from the advanced lines and the gene bank entries, and the latter were not well resolved.
AB - Cleome gynandra (L.) Briq. is an African leafy vegetable with a potential to improve food security and micronutrient deficiencies. Cytological traits, breeding biology and genetic diversity of 30 selected entries of C. gynandra from six African countries were investigated. The entries consisted of advanced lines, gene bank accessions and farmers’ cultivars. Our study revealed chromosome numbers of 2n = 34 in root tip metaphase cells from one entry. The 30 entries were found to be diploid with genome sizes ranging from 2.31 to 2.45 pg/2C. Hand pollination experiments were carried out to assess self-incompatibility within the entries and revealed that they are self- and cross-compatible. For genetic diversity studies within and among the entries, the pooled data of 499 polymorphic bands from 11 amplified fragment length polymorphism primer combinations and nine simple sequence repeat markers were used. The genetic distance among the entries ranged from 0.13 to 0.77. In a principal coordinate analysis, the farmers’ cultivars formed a cluster separate from the advanced lines and the gene bank entries, and the latter were not well resolved.
KW - amplified fragment length polymorphism
KW - breeding biology
KW - chromosome numbers
KW - flow cytometry
KW - gene bank accessions
KW - simple sequence repeat
UR - http://www.scopus.com/inward/record.url?scp=85019731631&partnerID=8YFLogxK
U2 - 10.1111/pbr.12485
DO - 10.1111/pbr.12485
M3 - Article
AN - SCOPUS:85019731631
VL - 136
SP - 578
EP - 589
JO - Plant Breeding
JF - Plant Breeding
SN - 0179-9541
IS - 4
ER -