Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 137-144 |
| Seitenumfang | 8 |
| Fachzeitschrift | Experimental Eye Research |
| Jahrgang | 146 |
| Publikationsstatus | Veröffentlicht - 8 Dez. 2015 |
Abstract
Two-photon microscopy (TPM) allows high contrast imaging at a subcellular resolution scale. In this work, the microscopy technique was applied to visualize corneal structures in two mouse models (BALB/c and B6.Cg-Tg(Thy1-YFP)16Jrs/J) in vivo. In particular, the transgenic Thy1-YFP mice expressing the yellow fluorescent protein (YFP) in all motor and sensory neurons had been used for investigating the nerve fiber density in healthy and streptozotocin-diabetic mice. This model is clinically relevant since patients suffering from diabetes mellitus have a high risk to develop small fiber neuropathy. Nonlinear laser scanning microscopy displayed a reduction of nerve fiber density in streptozotocin-diabetic versus healthy mice and confirmed data obtained by confocal laser scanning microscopy (CLSM). In recent years, corneal CLSM was proved to be an appropriate non-invasive tool for an early diagnosis of diabetic neuropathy. Nevertheless, validation of the CLSM method for the clinical routine is currently a matter of investigation and requires confirmation by further studies and complementary techniques. Thus, the present study provides further evidence of corneal confocal microscopy as a promising technique for non-invasive detection of diabetic neuropathy. Information derived from these experiments may become clinically relevant and help to develop new drugs for treatment of diabetic neuropathy.
ASJC Scopus Sachgebiete
- Medizin (insg.)
- Ophthalmologie
- Neurowissenschaften (insg.)
- Sensorische Systeme
- Neurowissenschaften (insg.)
- Zelluläre und Molekulare Neurowissenschaften
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in: Experimental Eye Research, Jahrgang 146, 08.12.2015, S. 137-144.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - In vivo nonlinear imaging of corneal structures with special focus on BALB/c and streptozotocin-diabetic Thy1-YFP mice
AU - Ehmke, Tobias
AU - Leckelt, Janine
AU - Reichard, Maria
AU - Weiss, Heike
AU - Hovakimyan, Marina
AU - Heisterkamp, Alexander
AU - Stachs, Oliver
AU - Baltrusch, Simone
N1 - Funding information: This work was supported by a grant from the German Diabetes Association (DDG) and by the German Federal Ministry of Education and Research (REMEDIS).
PY - 2015/12/8
Y1 - 2015/12/8
N2 - Two-photon microscopy (TPM) allows high contrast imaging at a subcellular resolution scale. In this work, the microscopy technique was applied to visualize corneal structures in two mouse models (BALB/c and B6.Cg-Tg(Thy1-YFP)16Jrs/J) in vivo. In particular, the transgenic Thy1-YFP mice expressing the yellow fluorescent protein (YFP) in all motor and sensory neurons had been used for investigating the nerve fiber density in healthy and streptozotocin-diabetic mice. This model is clinically relevant since patients suffering from diabetes mellitus have a high risk to develop small fiber neuropathy. Nonlinear laser scanning microscopy displayed a reduction of nerve fiber density in streptozotocin-diabetic versus healthy mice and confirmed data obtained by confocal laser scanning microscopy (CLSM). In recent years, corneal CLSM was proved to be an appropriate non-invasive tool for an early diagnosis of diabetic neuropathy. Nevertheless, validation of the CLSM method for the clinical routine is currently a matter of investigation and requires confirmation by further studies and complementary techniques. Thus, the present study provides further evidence of corneal confocal microscopy as a promising technique for non-invasive detection of diabetic neuropathy. Information derived from these experiments may become clinically relevant and help to develop new drugs for treatment of diabetic neuropathy.
AB - Two-photon microscopy (TPM) allows high contrast imaging at a subcellular resolution scale. In this work, the microscopy technique was applied to visualize corneal structures in two mouse models (BALB/c and B6.Cg-Tg(Thy1-YFP)16Jrs/J) in vivo. In particular, the transgenic Thy1-YFP mice expressing the yellow fluorescent protein (YFP) in all motor and sensory neurons had been used for investigating the nerve fiber density in healthy and streptozotocin-diabetic mice. This model is clinically relevant since patients suffering from diabetes mellitus have a high risk to develop small fiber neuropathy. Nonlinear laser scanning microscopy displayed a reduction of nerve fiber density in streptozotocin-diabetic versus healthy mice and confirmed data obtained by confocal laser scanning microscopy (CLSM). In recent years, corneal CLSM was proved to be an appropriate non-invasive tool for an early diagnosis of diabetic neuropathy. Nevertheless, validation of the CLSM method for the clinical routine is currently a matter of investigation and requires confirmation by further studies and complementary techniques. Thus, the present study provides further evidence of corneal confocal microscopy as a promising technique for non-invasive detection of diabetic neuropathy. Information derived from these experiments may become clinically relevant and help to develop new drugs for treatment of diabetic neuropathy.
KW - Applied optics
KW - Confocal microscopy
KW - Cornea
KW - Diabetic neuropathy
KW - Nonlinear microscopy
KW - Ophthalmology
UR - http://www.scopus.com/inward/record.url?scp=84954493120&partnerID=8YFLogxK
U2 - 10.1016/j.exer.2015.11.024
DO - 10.1016/j.exer.2015.11.024
M3 - Article
C2 - 26677758
AN - SCOPUS:84954493120
VL - 146
SP - 137
EP - 144
JO - Experimental Eye Research
JF - Experimental Eye Research
SN - 0014-4835
ER -