Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 975-983 |
| Seitenumfang | 9 |
| Fachzeitschrift | Journal of Applied Physiology |
| Jahrgang | 113 |
| Ausgabenummer | 6 |
| Publikationsstatus | Veröffentlicht - 15 Sept. 2012 |
| Extern publiziert | Ja |
Abstract
The current study focuses on the use of scanning laser optical tomography (SLOT) in imaging of the mouse lung ex vivo. SLOT is a highly efficient fluorescence microscopy technique allowing rapid scanning of samples of a size of several millimeters, thus enabling volumetric visualization by using intrinsic contrast mechanisms of previously fixed lung lobes. Here, we demonstrate the imaging of airways, blood vessels, and parenchyma from whole, optically cleared mouse lung lobes with a resolution down to the level of single alveoli using absorption and autofluorescence scan modes. The internal structure of the lung can then be analyzed nondestructively and quantitatively in three-dimensional datasets in any preferred planar orientation. Moreover, the procedure preserves the microscopic structure of the lung and allows for subsequent correlative histologic studies. In summary, the current study has shown that SLOT is a valuable technique to study the internal structure of the mouse lung.
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Physiologie
- Medizin (insg.)
- Physiologie (medizinische)
Zitieren
- Standard
- Harvard
- Apa
- Vancouver
- BibTex
- RIS
in: Journal of Applied Physiology, Jahrgang 113, Nr. 6, 15.09.2012, S. 975-983.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Imaging of the mouse lung with scanning laser optical tomography (SLOT)
AU - Kellner, Manuela
AU - Heidrich, Marko
AU - Beigel, Rebecca
AU - Lorbeer, Raoul Amadeus
AU - Knudsen, Lars
AU - Ripken, Tammo
AU - Heisterkamp, Alexander
AU - Meyer, Heiko
AU - Kühnel, Mark Philipp
AU - Ochs, Matthias
PY - 2012/9/15
Y1 - 2012/9/15
N2 - The current study focuses on the use of scanning laser optical tomography (SLOT) in imaging of the mouse lung ex vivo. SLOT is a highly efficient fluorescence microscopy technique allowing rapid scanning of samples of a size of several millimeters, thus enabling volumetric visualization by using intrinsic contrast mechanisms of previously fixed lung lobes. Here, we demonstrate the imaging of airways, blood vessels, and parenchyma from whole, optically cleared mouse lung lobes with a resolution down to the level of single alveoli using absorption and autofluorescence scan modes. The internal structure of the lung can then be analyzed nondestructively and quantitatively in three-dimensional datasets in any preferred planar orientation. Moreover, the procedure preserves the microscopic structure of the lung and allows for subsequent correlative histologic studies. In summary, the current study has shown that SLOT is a valuable technique to study the internal structure of the mouse lung.
AB - The current study focuses on the use of scanning laser optical tomography (SLOT) in imaging of the mouse lung ex vivo. SLOT is a highly efficient fluorescence microscopy technique allowing rapid scanning of samples of a size of several millimeters, thus enabling volumetric visualization by using intrinsic contrast mechanisms of previously fixed lung lobes. Here, we demonstrate the imaging of airways, blood vessels, and parenchyma from whole, optically cleared mouse lung lobes with a resolution down to the level of single alveoli using absorption and autofluorescence scan modes. The internal structure of the lung can then be analyzed nondestructively and quantitatively in three-dimensional datasets in any preferred planar orientation. Moreover, the procedure preserves the microscopic structure of the lung and allows for subsequent correlative histologic studies. In summary, the current study has shown that SLOT is a valuable technique to study the internal structure of the mouse lung.
KW - 3D microscopy
KW - Acini
KW - Airways
KW - Alveoli
KW - Blood vessels
KW - Optical projection tomography
UR - http://www.scopus.com/inward/record.url?scp=84866381198&partnerID=8YFLogxK
U2 - 10.1152/japplphysiol.00026.2012
DO - 10.1152/japplphysiol.00026.2012
M3 - Article
C2 - 22797312
AN - SCOPUS:84866381198
VL - 113
SP - 975
EP - 983
JO - Journal of Applied Physiology
JF - Journal of Applied Physiology
SN - 8750-7587
IS - 6
ER -