Hypothermic preservation of endothelialized gas-exchange membranes

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autoren

  • Michael Pflaum
  • Hayan Merhej
  • Ariana Peredo
  • Adim De
  • Daniele Dipresa
  • Bettina Wiegmann
  • Willem Wolkers
  • Axel Haverich
  • Sotirios Korossis

Organisationseinheiten

Externe Organisationen

  • Medizinische Hochschule Hannover (MHH)
  • Stiftung Tierärztliche Hochschule Hannover
  • Loughborough University
Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Seiten (von - bis)e552-e565
FachzeitschriftArtificial Organs
Jahrgang44
Ausgabenummer12
Frühes Online-Datum15 Juli 2020
PublikationsstatusVeröffentlicht - 17 Dez. 2020

Abstract

Endothelialization of the blood contacting surfaces of blood-contacting medical devices, such as cardiovascular prostheses or biohybrid oxygenators, represents a plausible strategy for increasing their hemocompatibility. Nevertheless, isolation and expansion of autologous endothelial cells (ECs) usually requires multiple processing steps and time to obtain sufficient cell numbers. This excludes endothelialization from application in acute situations. Off-the-shelf availability of cell-seeded biohybrid devices could be potentially facilitated by hypothermic storage. In this study, the survival of cord-blood-derived endothelial colony forming cells (ECFCs) that were seeded onto polymethylpentene (PMP) gas-exchange membranes and stored for up to 2 weeks in different commercially available and commonly used preservation media was measured. While storage at 4°C in normal growth medium (EGM-2) for 3 days resulted in massive disruption of the ECFC monolayer and a significant decline in viability, ECFC monolayers preserved in Chillprotec could recover after up to 14 days with negligible effects on their integrity and viability. ECFC monolayers preserved in Celsior, HTS-FRS, or Rokepie medium showed a significant decrease in viability after 7 days or longer periods. These results demonstrated the feasibility of hypothermic preservation of ECFC monolayers on gas-exchange membranes for up to 2 weeks, with potential application on the preservation of pre-endothelialized oxygenators and further biohybrid cardiovascular devices.

ASJC Scopus Sachgebiete

Zitieren

Hypothermic preservation of endothelialized gas-exchange membranes. / Pflaum, Michael; Merhej, Hayan; Peredo, Ariana et al.
in: Artificial Organs, Jahrgang 44, Nr. 12, 17.12.2020, S. e552-e565.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Pflaum, M, Merhej, H, Peredo, A, De, A, Dipresa, D, Wiegmann, B, Wolkers, W, Haverich, A & Korossis, S 2020, 'Hypothermic preservation of endothelialized gas-exchange membranes', Artificial Organs, Jg. 44, Nr. 12, S. e552-e565. https://doi.org/10.1111/aor.13776
Pflaum, M., Merhej, H., Peredo, A., De, A., Dipresa, D., Wiegmann, B., Wolkers, W., Haverich, A., & Korossis, S. (2020). Hypothermic preservation of endothelialized gas-exchange membranes. Artificial Organs, 44(12), e552-e565. https://doi.org/10.1111/aor.13776
Pflaum M, Merhej H, Peredo A, De A, Dipresa D, Wiegmann B et al. Hypothermic preservation of endothelialized gas-exchange membranes. Artificial Organs. 2020 Dez 17;44(12):e552-e565. Epub 2020 Jul 15. doi: 10.1111/aor.13776
Pflaum, Michael ; Merhej, Hayan ; Peredo, Ariana et al. / Hypothermic preservation of endothelialized gas-exchange membranes. in: Artificial Organs. 2020 ; Jahrgang 44, Nr. 12. S. e552-e565.
Download
@article{19a13a6d28f04b97817e7396aa0760ed,
title = "Hypothermic preservation of endothelialized gas-exchange membranes",
abstract = "Endothelialization of the blood contacting surfaces of blood-contacting medical devices, such as cardiovascular prostheses or biohybrid oxygenators, represents a plausible strategy for increasing their hemocompatibility. Nevertheless, isolation and expansion of autologous endothelial cells (ECs) usually requires multiple processing steps and time to obtain sufficient cell numbers. This excludes endothelialization from application in acute situations. Off-the-shelf availability of cell-seeded biohybrid devices could be potentially facilitated by hypothermic storage. In this study, the survival of cord-blood-derived endothelial colony forming cells (ECFCs) that were seeded onto polymethylpentene (PMP) gas-exchange membranes and stored for up to 2 weeks in different commercially available and commonly used preservation media was measured. While storage at 4°C in normal growth medium (EGM-2) for 3 days resulted in massive disruption of the ECFC monolayer and a significant decline in viability, ECFC monolayers preserved in Chillprotec could recover after up to 14 days with negligible effects on their integrity and viability. ECFC monolayers preserved in Celsior, HTS-FRS, or Rokepie medium showed a significant decrease in viability after 7 days or longer periods. These results demonstrated the feasibility of hypothermic preservation of ECFC monolayers on gas-exchange membranes for up to 2 weeks, with potential application on the preservation of pre-endothelialized oxygenators and further biohybrid cardiovascular devices.",
keywords = "bioartificial lung, biohybrid lung, biohybrid organs, endothelialization, hypothermic preservation",
author = "Michael Pflaum and Hayan Merhej and Ariana Peredo and Adim De and Daniele Dipresa and Bettina Wiegmann and Willem Wolkers and Axel Haverich and Sotirios Korossis",
note = "Funding Information: This study was supported in part by the Cluster of Excellence REBIRTH (From Regenerative Biology to Reconstructive Therapy EXC62: 24102914), the German Centre for Lung Research (DZL) BREATH (Biomedical Research In Endstage And Obstructive Lung Disease Hannover; DZL: 82DZL00201), the German Research Foundation (DFG; Projects WI 4088/1‐2, SPP2014: 347346497 and SPP2014: 348028075). ",
year = "2020",
month = dec,
day = "17",
doi = "10.1111/aor.13776",
language = "English",
volume = "44",
pages = "e552--e565",
journal = "Artificial Organs",
issn = "0160-564X",
publisher = "Wiley-Blackwell Publishing Ltd",
number = "12",

}

Download

TY - JOUR

T1 - Hypothermic preservation of endothelialized gas-exchange membranes

AU - Pflaum, Michael

AU - Merhej, Hayan

AU - Peredo, Ariana

AU - De, Adim

AU - Dipresa, Daniele

AU - Wiegmann, Bettina

AU - Wolkers, Willem

AU - Haverich, Axel

AU - Korossis, Sotirios

N1 - Funding Information: This study was supported in part by the Cluster of Excellence REBIRTH (From Regenerative Biology to Reconstructive Therapy EXC62: 24102914), the German Centre for Lung Research (DZL) BREATH (Biomedical Research In Endstage And Obstructive Lung Disease Hannover; DZL: 82DZL00201), the German Research Foundation (DFG; Projects WI 4088/1‐2, SPP2014: 347346497 and SPP2014: 348028075).

PY - 2020/12/17

Y1 - 2020/12/17

N2 - Endothelialization of the blood contacting surfaces of blood-contacting medical devices, such as cardiovascular prostheses or biohybrid oxygenators, represents a plausible strategy for increasing their hemocompatibility. Nevertheless, isolation and expansion of autologous endothelial cells (ECs) usually requires multiple processing steps and time to obtain sufficient cell numbers. This excludes endothelialization from application in acute situations. Off-the-shelf availability of cell-seeded biohybrid devices could be potentially facilitated by hypothermic storage. In this study, the survival of cord-blood-derived endothelial colony forming cells (ECFCs) that were seeded onto polymethylpentene (PMP) gas-exchange membranes and stored for up to 2 weeks in different commercially available and commonly used preservation media was measured. While storage at 4°C in normal growth medium (EGM-2) for 3 days resulted in massive disruption of the ECFC monolayer and a significant decline in viability, ECFC monolayers preserved in Chillprotec could recover after up to 14 days with negligible effects on their integrity and viability. ECFC monolayers preserved in Celsior, HTS-FRS, or Rokepie medium showed a significant decrease in viability after 7 days or longer periods. These results demonstrated the feasibility of hypothermic preservation of ECFC monolayers on gas-exchange membranes for up to 2 weeks, with potential application on the preservation of pre-endothelialized oxygenators and further biohybrid cardiovascular devices.

AB - Endothelialization of the blood contacting surfaces of blood-contacting medical devices, such as cardiovascular prostheses or biohybrid oxygenators, represents a plausible strategy for increasing their hemocompatibility. Nevertheless, isolation and expansion of autologous endothelial cells (ECs) usually requires multiple processing steps and time to obtain sufficient cell numbers. This excludes endothelialization from application in acute situations. Off-the-shelf availability of cell-seeded biohybrid devices could be potentially facilitated by hypothermic storage. In this study, the survival of cord-blood-derived endothelial colony forming cells (ECFCs) that were seeded onto polymethylpentene (PMP) gas-exchange membranes and stored for up to 2 weeks in different commercially available and commonly used preservation media was measured. While storage at 4°C in normal growth medium (EGM-2) for 3 days resulted in massive disruption of the ECFC monolayer and a significant decline in viability, ECFC monolayers preserved in Chillprotec could recover after up to 14 days with negligible effects on their integrity and viability. ECFC monolayers preserved in Celsior, HTS-FRS, or Rokepie medium showed a significant decrease in viability after 7 days or longer periods. These results demonstrated the feasibility of hypothermic preservation of ECFC monolayers on gas-exchange membranes for up to 2 weeks, with potential application on the preservation of pre-endothelialized oxygenators and further biohybrid cardiovascular devices.

KW - bioartificial lung

KW - biohybrid lung

KW - biohybrid organs

KW - endothelialization

KW - hypothermic preservation

UR - http://www.scopus.com/inward/record.url?scp=85089391652&partnerID=8YFLogxK

U2 - 10.1111/aor.13776

DO - 10.1111/aor.13776

M3 - Article

C2 - 32666514

AN - SCOPUS:85089391652

VL - 44

SP - e552-e565

JO - Artificial Organs

JF - Artificial Organs

SN - 0160-564X

IS - 12

ER -