TY - JOUR

T1 - Exploring Sulfate as an Alternative Electron Acceptor

T2 - A Potential Strategy to Mitigate N2O Emissions in Upland Arable Soils.

AU - Lee, Hyun Ho

AU - Kim, Hanbeen

AU - Park, Ye Lim

AU - Horn, Marcus A

AU - Kim, Jeongeun

AU - Lee, Jaehyun

AU - Toyoda, Sakae

AU - Yun, Jeongeun

AU - Kang, Hojeong

AU - Kim, Sang Yoon

AU - Ahn, Jinho

AU - Hong, Chang Oh

N1 - © 2025 The Author(s). Global Change Biology published by John Wiley & Sons Ltd.

PY - 2025/8/13

Y1 - 2025/8/13

N2 - Agricultural activities are a significant source of nitrous oxide (N 2O), accounting for approximately 60% of global emissions, highlighting the urgent need for innovative strategies to mitigate N 2O emissions. Microbes conserve nearly as much energy with nitrate (NO 3 -) as oxygen (O 2) respiration under limited O 2 availability. Thus, microorganisms prioritize NO 3 -, limiting exploration of alternative electron acceptors (EAs) to inhibit N 2O emissions through NO 3 - respiration in upland arable soils. Current approaches remain insufficient, and the interactions between alternative EA reduction and pathways for N 2O emissions remain poorly understood. This study evaluated oxidized iron, manganese, and sulfate as alternative EAs to reduce N 2O emissions, along with the effects of zero-valent metals (ZVMs). Metal sulfates (MSs) significantly minimized N 2O emissions by inhibiting denitrification rather than altering nitrification in microcosms, as supported by isotope mapping and inorganic nitrogen concentrations. Among others, putative complete denitrifiers, N 2O reducers, and sulfate reducers were stimulated, whereas ZVMs stimulated N 2O emissions and 16S rRNA gene abundance. Moreover, the abundance of denitrifier-related genes (nirK, nirS, norB, and nosZ) consistently decreased under MS treatments, while dsrA mRNA abundance significantly increased. Sulfate (SO 4 2-) addition reshaped the soil microbial community by enriching sulfur-cycling taxa-including sulfate-reducing and sulfur-oxidizing bacteria-while suppressing nitrifiers such as Nitrospira, potentially disrupting nitrification-denitrification coupling. Ureibacillus thermosphaerius, harboring genes for denitrification and SO 4 2- reduction, increased under MS treatment. These shifts likely redirected electron flow toward SO 4 2- respiration, reducing NO 3 - utilization and contributing to N 2O mitigation. Field-based manipulation experiments over 2 years demonstrated the feasibility of MSs in upland arable soils, reducing yield-scaled N 2O emissions by 21.5% without compromising crop yields. A systematic literature review and meta-analysis revealed that SO 4 2- application mitigated N 2O emissions by an average of 9%, with over 70% of observations showing a decreasing trend, underscoring its potential as an effective soil amendment for sustainable agriculture.

AB - Agricultural activities are a significant source of nitrous oxide (N 2O), accounting for approximately 60% of global emissions, highlighting the urgent need for innovative strategies to mitigate N 2O emissions. Microbes conserve nearly as much energy with nitrate (NO 3 -) as oxygen (O 2) respiration under limited O 2 availability. Thus, microorganisms prioritize NO 3 -, limiting exploration of alternative electron acceptors (EAs) to inhibit N 2O emissions through NO 3 - respiration in upland arable soils. Current approaches remain insufficient, and the interactions between alternative EA reduction and pathways for N 2O emissions remain poorly understood. This study evaluated oxidized iron, manganese, and sulfate as alternative EAs to reduce N 2O emissions, along with the effects of zero-valent metals (ZVMs). Metal sulfates (MSs) significantly minimized N 2O emissions by inhibiting denitrification rather than altering nitrification in microcosms, as supported by isotope mapping and inorganic nitrogen concentrations. Among others, putative complete denitrifiers, N 2O reducers, and sulfate reducers were stimulated, whereas ZVMs stimulated N 2O emissions and 16S rRNA gene abundance. Moreover, the abundance of denitrifier-related genes (nirK, nirS, norB, and nosZ) consistently decreased under MS treatments, while dsrA mRNA abundance significantly increased. Sulfate (SO 4 2-) addition reshaped the soil microbial community by enriching sulfur-cycling taxa-including sulfate-reducing and sulfur-oxidizing bacteria-while suppressing nitrifiers such as Nitrospira, potentially disrupting nitrification-denitrification coupling. Ureibacillus thermosphaerius, harboring genes for denitrification and SO 4 2- reduction, increased under MS treatment. These shifts likely redirected electron flow toward SO 4 2- respiration, reducing NO 3 - utilization and contributing to N 2O mitigation. Field-based manipulation experiments over 2 years demonstrated the feasibility of MSs in upland arable soils, reducing yield-scaled N 2O emissions by 21.5% without compromising crop yields. A systematic literature review and meta-analysis revealed that SO 4 2- application mitigated N 2O emissions by an average of 9%, with over 70% of observations showing a decreasing trend, underscoring its potential as an effective soil amendment for sustainable agriculture.

KW - Denitrification

KW - Nitrous oxide

KW - Sulfate reduction

KW - Terminal electron acceptors

KW - Upland arable soils

UR - http://www.scopus.com/inward/record.url?scp=105013387378&partnerID=8YFLogxK

U2 - 10.1111/gcb.70428

DO - 10.1111/gcb.70428

M3 - Article

C2 - 40801137

VL - 31

JO - Global change biology

JF - Global change biology

SN - 1354-1013

IS - 8

M1 - e70428

ER -