Doublesex regulates male-specific differentiation during distinct developmental time windows in a parasitoid wasp

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autorschaft

  • Yidong Wang
  • Anna Rensink
  • Ute Fricke
  • Megan C. Riddle
  • Carol Trent
  • Louis van de Zande
  • Eveline C. Verhulst

Externe Organisationen

  • Wageningen University and Research
  • Reichsuniversität Groningen
  • Western Washington University
Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Aufsatznummer103724
Seitenumfang11
FachzeitschriftInsect Biochemistry and Molecular Biology
Jahrgang142
Frühes Online-Datum29 Jan. 2022
PublikationsstatusVeröffentlicht - März 2022
Extern publiziertJa

Abstract

Sexually dimorphic traits in insects are subject to sexual selection, but our knowledge of the underlying molecular mechanisms is still scarce. Here we investigate how the highly conserved gene, Doublesex (Dsx), is involved in shaping sexual dimorphism in the model parasitoid wasp Nasonia vitripennis (Hymenoptera: Pteromalidae). First, we present the revised Dsx gene structure including an alternative transcription start, and two additional male NvDsx transcript isoforms. We show sex-specific NvDsx expression and splicing throughout development, and demonstrate that transient NvDsx silencing in different male developmental stages shifts two sexually dimorphic traits from male to female morphology, with the effect being dependent on the timing of silencing. In addition, we determined the effect of NvDsx on the development of reproductive organs. Transient silencing of NvDsx in early male larvae affects the growth and differentiation of the internal and external reproductive tissues. We did not observe phenotypic changes in females after NvDsx silencing. Our results indicate that male NvDsx is required to suppress female-specific traits and/or to promote male-specific traits during distinct developmental windows. This provides new insights into the regulatory activity of Dsx during male wasp development in the Hymenoptera.

ASJC Scopus Sachgebiete

Zitieren

Doublesex regulates male-specific differentiation during distinct developmental time windows in a parasitoid wasp. / Wang, Yidong; Rensink, Anna; Fricke, Ute et al.
in: Insect Biochemistry and Molecular Biology, Jahrgang 142, 103724, 03.2022.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Wang Y, Rensink A, Fricke U, Riddle MC, Trent C, Zande LVD et al. Doublesex regulates male-specific differentiation during distinct developmental time windows in a parasitoid wasp. Insect Biochemistry and Molecular Biology. 2022 Mär;142:103724. Epub 2022 Jan 29. doi: 10.1016/j.ibmb.2022.103724, 10.1101/2020.04.19.048553, 10.1016/j.ibmb.2022.103724
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abstract = "Sexually dimorphic traits in insects are subject to sexual selection, but our knowledge of the underlying molecular mechanisms is still scarce. Here we investigate how the highly conserved gene, Doublesex (Dsx), is involved in shaping sexual dimorphism in the model parasitoid wasp Nasonia vitripennis (Hymenoptera: Pteromalidae). First, we present the revised Dsx gene structure including an alternative transcription start, and two additional male NvDsx transcript isoforms. We show sex-specific NvDsx expression and splicing throughout development, and demonstrate that transient NvDsx silencing in different male developmental stages shifts two sexually dimorphic traits from male to female morphology, with the effect being dependent on the timing of silencing. In addition, we determined the effect of NvDsx on the development of reproductive organs. Transient silencing of NvDsx in early male larvae affects the growth and differentiation of the internal and external reproductive tissues. We did not observe phenotypic changes in females after NvDsx silencing. Our results indicate that male NvDsx is required to suppress female-specific traits and/or to promote male-specific traits during distinct developmental windows. This provides new insights into the regulatory activity of Dsx during male wasp development in the Hymenoptera.",
keywords = "Doublesex, Nasonia, Parasitoid wasps, Sex determination, Sexual differentiation, Sexually dimorphic traits",
author = "Yidong Wang and Anna Rensink and Ute Fricke and Riddle, {Megan C.} and Carol Trent and Zande, {Louis van de} and Verhulst, {Eveline C.}",
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Download

TY - JOUR

T1 - Doublesex regulates male-specific differentiation during distinct developmental time windows in a parasitoid wasp

AU - Wang, Yidong

AU - Rensink, Anna

AU - Fricke, Ute

AU - Riddle, Megan C.

AU - Trent, Carol

AU - Zande, Louis van de

AU - Verhulst, Eveline C.

N1 - Publisher Copyright: © 2022 The Authors

PY - 2022/3

Y1 - 2022/3

N2 - Sexually dimorphic traits in insects are subject to sexual selection, but our knowledge of the underlying molecular mechanisms is still scarce. Here we investigate how the highly conserved gene, Doublesex (Dsx), is involved in shaping sexual dimorphism in the model parasitoid wasp Nasonia vitripennis (Hymenoptera: Pteromalidae). First, we present the revised Dsx gene structure including an alternative transcription start, and two additional male NvDsx transcript isoforms. We show sex-specific NvDsx expression and splicing throughout development, and demonstrate that transient NvDsx silencing in different male developmental stages shifts two sexually dimorphic traits from male to female morphology, with the effect being dependent on the timing of silencing. In addition, we determined the effect of NvDsx on the development of reproductive organs. Transient silencing of NvDsx in early male larvae affects the growth and differentiation of the internal and external reproductive tissues. We did not observe phenotypic changes in females after NvDsx silencing. Our results indicate that male NvDsx is required to suppress female-specific traits and/or to promote male-specific traits during distinct developmental windows. This provides new insights into the regulatory activity of Dsx during male wasp development in the Hymenoptera.

AB - Sexually dimorphic traits in insects are subject to sexual selection, but our knowledge of the underlying molecular mechanisms is still scarce. Here we investigate how the highly conserved gene, Doublesex (Dsx), is involved in shaping sexual dimorphism in the model parasitoid wasp Nasonia vitripennis (Hymenoptera: Pteromalidae). First, we present the revised Dsx gene structure including an alternative transcription start, and two additional male NvDsx transcript isoforms. We show sex-specific NvDsx expression and splicing throughout development, and demonstrate that transient NvDsx silencing in different male developmental stages shifts two sexually dimorphic traits from male to female morphology, with the effect being dependent on the timing of silencing. In addition, we determined the effect of NvDsx on the development of reproductive organs. Transient silencing of NvDsx in early male larvae affects the growth and differentiation of the internal and external reproductive tissues. We did not observe phenotypic changes in females after NvDsx silencing. Our results indicate that male NvDsx is required to suppress female-specific traits and/or to promote male-specific traits during distinct developmental windows. This provides new insights into the regulatory activity of Dsx during male wasp development in the Hymenoptera.

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KW - Nasonia

KW - Parasitoid wasps

KW - Sex determination

KW - Sexual differentiation

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