Discrepancy in exchangeable and soluble ammonium-induced effects on aerobic methane oxidation: a microcosm study of a paddy soil

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autoren

Organisationseinheiten

Externe Organisationen

  • Kunsan National University
  • Netherlands Institute of Ecology
Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Seiten (von - bis)873-880
Seitenumfang8
FachzeitschriftBiology and fertility of soils
Jahrgang57
Ausgabenummer6
Frühes Online-Datum22 Juni 2021
PublikationsstatusVeröffentlicht - Aug. 2021

Abstract

Ammonium-induced stimulatory, inhibitory, and/or neutral effects on soil methane oxidation have been attributable to the ammonium concentration and mineral forms, confounded by other edaphic properties (e.g., pH, salinity), as well as the site-specific composition of the methanotrophic community. We hypothesize that this inconsistency may stem from the discrepancy in the cation adsorption capacity of the soil. We postulate that the effects of ammonium on the methanotrophic activity in soil are more accurately portrayed by relating methane uptake rates to the soluble ammonium (bioavailable), rather than the exchangeable (total) ammonium. To reduce adsorption (exchangeable) sites for ammonium in a paddy soil, two successive pre-incubation steps were introduced resulting in a 1000-fold soil dilution (soil enrichment), to be compared to a soil slurry (tenfold dilution) incubation. Ammonium was supplemented as NH 4Cl at 0.5–4.75gL −1 after pre-incubation. While NH 4Cl significantly stimulated the methanotrophic activity at all concentrations in the soil slurry incubation, methane uptake showed a dose-dependent effect in the soil enrichment. The trend in methane uptake could be explained by the soluble ammonium concentration, which was proportionate to the supplemented ammonium in the soil enrichment. In the soil slurry incubation, a fraction (36–63%) of the supplemented ammonium was determined to be adsorbed to the soil. Accordingly, Methylosarcina was found to predominate the methanotrophic community after the incubation, suggesting the relevance of this methanotroph at elevated ammonium levels (< 3.25gL −1 NH 4Cl). Collectively, our results showed that the soluble, rather than the exchangeable ammonium concentration, is relevant when determining the effects of ammonium on methane oxidation, but this does not exclude other (a)biotic factors concurrently influencing methanotrophic activity.

ASJC Scopus Sachgebiete

Zitieren

Discrepancy in exchangeable and soluble ammonium-induced effects on aerobic methane oxidation: a microcosm study of a paddy soil. / van Dijk, Hester; Kaupper, Thomas; Bothe, Clemens et al.
in: Biology and fertility of soils, Jahrgang 57, Nr. 6, 08.2021, S. 873-880.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

van Dijk H, Kaupper T, Bothe C, Lee HJ, Bodelier PLE, Horn MA et al. Discrepancy in exchangeable and soluble ammonium-induced effects on aerobic methane oxidation: a microcosm study of a paddy soil. Biology and fertility of soils. 2021 Aug;57(6):873-880. Epub 2021 Jun 22. doi: 10.1007/s00374-021-01579-9
Download
@article{d2b37b6283014f0abc5aa230928fb8e6,
title = "Discrepancy in exchangeable and soluble ammonium-induced effects on aerobic methane oxidation: a microcosm study of a paddy soil",
abstract = "Ammonium-induced stimulatory, inhibitory, and/or neutral effects on soil methane oxidation have been attributable to the ammonium concentration and mineral forms, confounded by other edaphic properties (e.g., pH, salinity), as well as the site-specific composition of the methanotrophic community. We hypothesize that this inconsistency may stem from the discrepancy in the cation adsorption capacity of the soil. We postulate that the effects of ammonium on the methanotrophic activity in soil are more accurately portrayed by relating methane uptake rates to the soluble ammonium (bioavailable), rather than the exchangeable (total) ammonium. To reduce adsorption (exchangeable) sites for ammonium in a paddy soil, two successive pre-incubation steps were introduced resulting in a 1000-fold soil dilution (soil enrichment), to be compared to a soil slurry (tenfold dilution) incubation. Ammonium was supplemented as NH 4Cl at 0.5–4.75gL −1 after pre-incubation. While NH 4Cl significantly stimulated the methanotrophic activity at all concentrations in the soil slurry incubation, methane uptake showed a dose-dependent effect in the soil enrichment. The trend in methane uptake could be explained by the soluble ammonium concentration, which was proportionate to the supplemented ammonium in the soil enrichment. In the soil slurry incubation, a fraction (36–63%) of the supplemented ammonium was determined to be adsorbed to the soil. Accordingly, Methylosarcina was found to predominate the methanotrophic community after the incubation, suggesting the relevance of this methanotroph at elevated ammonium levels (< 3.25gL −1 NH 4Cl). Collectively, our results showed that the soluble, rather than the exchangeable ammonium concentration, is relevant when determining the effects of ammonium on methane oxidation, but this does not exclude other (a)biotic factors concurrently influencing methanotrophic activity. ",
keywords = "Ammonium-based fertilization, Methanotroph, Methylosarcina, pmoA, Wetland agriculture",
author = "{van Dijk}, Hester and Thomas Kaupper and Clemens Bothe and Lee, {Hyo Jung} and Bodelier, {Paul L. E.} and Horn, {Marcus A.} and Adrian Ho",
note = "Funding Information: Open Access funding enabled and organized by Projekt DEAL. TK and AH are financially supported by the Deutsche Forschungsgemeinschaft (grant no. HO6234/1–1). AH and MAH are also financially supported by the Leibniz Universit{\"a}t Hannover, Germany.",
year = "2021",
month = aug,
doi = "10.1007/s00374-021-01579-9",
language = "English",
volume = "57",
pages = "873--880",
journal = "Biology and fertility of soils",
issn = "0178-2762",
publisher = "Springer Verlag",
number = "6",

}

Download

TY - JOUR

T1 - Discrepancy in exchangeable and soluble ammonium-induced effects on aerobic methane oxidation

T2 - a microcosm study of a paddy soil

AU - van Dijk, Hester

AU - Kaupper, Thomas

AU - Bothe, Clemens

AU - Lee, Hyo Jung

AU - Bodelier, Paul L. E.

AU - Horn, Marcus A.

AU - Ho, Adrian

N1 - Funding Information: Open Access funding enabled and organized by Projekt DEAL. TK and AH are financially supported by the Deutsche Forschungsgemeinschaft (grant no. HO6234/1–1). AH and MAH are also financially supported by the Leibniz Universität Hannover, Germany.

PY - 2021/8

Y1 - 2021/8

N2 - Ammonium-induced stimulatory, inhibitory, and/or neutral effects on soil methane oxidation have been attributable to the ammonium concentration and mineral forms, confounded by other edaphic properties (e.g., pH, salinity), as well as the site-specific composition of the methanotrophic community. We hypothesize that this inconsistency may stem from the discrepancy in the cation adsorption capacity of the soil. We postulate that the effects of ammonium on the methanotrophic activity in soil are more accurately portrayed by relating methane uptake rates to the soluble ammonium (bioavailable), rather than the exchangeable (total) ammonium. To reduce adsorption (exchangeable) sites for ammonium in a paddy soil, two successive pre-incubation steps were introduced resulting in a 1000-fold soil dilution (soil enrichment), to be compared to a soil slurry (tenfold dilution) incubation. Ammonium was supplemented as NH 4Cl at 0.5–4.75gL −1 after pre-incubation. While NH 4Cl significantly stimulated the methanotrophic activity at all concentrations in the soil slurry incubation, methane uptake showed a dose-dependent effect in the soil enrichment. The trend in methane uptake could be explained by the soluble ammonium concentration, which was proportionate to the supplemented ammonium in the soil enrichment. In the soil slurry incubation, a fraction (36–63%) of the supplemented ammonium was determined to be adsorbed to the soil. Accordingly, Methylosarcina was found to predominate the methanotrophic community after the incubation, suggesting the relevance of this methanotroph at elevated ammonium levels (< 3.25gL −1 NH 4Cl). Collectively, our results showed that the soluble, rather than the exchangeable ammonium concentration, is relevant when determining the effects of ammonium on methane oxidation, but this does not exclude other (a)biotic factors concurrently influencing methanotrophic activity.

AB - Ammonium-induced stimulatory, inhibitory, and/or neutral effects on soil methane oxidation have been attributable to the ammonium concentration and mineral forms, confounded by other edaphic properties (e.g., pH, salinity), as well as the site-specific composition of the methanotrophic community. We hypothesize that this inconsistency may stem from the discrepancy in the cation adsorption capacity of the soil. We postulate that the effects of ammonium on the methanotrophic activity in soil are more accurately portrayed by relating methane uptake rates to the soluble ammonium (bioavailable), rather than the exchangeable (total) ammonium. To reduce adsorption (exchangeable) sites for ammonium in a paddy soil, two successive pre-incubation steps were introduced resulting in a 1000-fold soil dilution (soil enrichment), to be compared to a soil slurry (tenfold dilution) incubation. Ammonium was supplemented as NH 4Cl at 0.5–4.75gL −1 after pre-incubation. While NH 4Cl significantly stimulated the methanotrophic activity at all concentrations in the soil slurry incubation, methane uptake showed a dose-dependent effect in the soil enrichment. The trend in methane uptake could be explained by the soluble ammonium concentration, which was proportionate to the supplemented ammonium in the soil enrichment. In the soil slurry incubation, a fraction (36–63%) of the supplemented ammonium was determined to be adsorbed to the soil. Accordingly, Methylosarcina was found to predominate the methanotrophic community after the incubation, suggesting the relevance of this methanotroph at elevated ammonium levels (< 3.25gL −1 NH 4Cl). Collectively, our results showed that the soluble, rather than the exchangeable ammonium concentration, is relevant when determining the effects of ammonium on methane oxidation, but this does not exclude other (a)biotic factors concurrently influencing methanotrophic activity.

KW - Ammonium-based fertilization

KW - Methanotroph

KW - Methylosarcina

KW - pmoA

KW - Wetland agriculture

UR - http://www.scopus.com/inward/record.url?scp=85108646253&partnerID=8YFLogxK

U2 - 10.1007/s00374-021-01579-9

DO - 10.1007/s00374-021-01579-9

M3 - Article

VL - 57

SP - 873

EP - 880

JO - Biology and fertility of soils

JF - Biology and fertility of soils

SN - 0178-2762

IS - 6

ER -

Von denselben Autoren