TY - JOUR

T1 - Differential impact of amino acids on OXPHOS system activity following carbohydrate starvation in Arabidopsis cell suspensions

AU - Cavalcanti, João Henrique F.

AU - Quinhones, Carla G.S.

AU - Schertl, Peter

AU - Brito, Danielle S.

AU - Eubel, Holger

AU - Hildebrandt, Tatjana

AU - Nunes-Nesi, Adriano

AU - Braun, Hans Peter

AU - Araújo, Wagner L.

N1 - Funding information: – We would like to thank Prof. Dr Traud Winkelmann (Leibniz Universität Hannover) for kindly providing her laboratory physical space and equipment for performing part of this research. Furthermore, we gratefully acknowledge Christa Ruppelt, Dagmar Lewejohann, and Marianne Langer for excellent technical assistance. This work was supported by supported by the binational science funding program ‘PROBRAL’ of the Deutsche Akademis-che Austauschdienst (DAAD; funds provided by the Bundesministerium für Bildung und Forschung – BMBF) and the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Refs: Project-ID PROBRAL #423/14. Research fellowships granted by the Foundation for Research Assistance of the Minas Gerais State (FAPEMIG-Brazil grant # BDP-00018-16) to J. H. F. C. and the National Council for Scientific and Technological Development (CNPq-Brazil) to A. N. N. and W. L. A. are gratefully acknowledged.

PY - 2017/8/2

Y1 - 2017/8/2

N2 - Plant respiration mostly depends on the activity of glycolysis and the oxidation of organic acids in the tricarboxylic acid cycle to synthesize ATP. However, during stress situations plant cells also use amino acids as alternative substrates to donate electrons through the electron-transfer flavoprotein (ETF)/ETF:ubiquinone oxidoreductase (ETF/ETFQO) complex to the mitochondrial electron transport chain (mETC). Given this, we investigated changes of the oxidative phosphorylation (OXPHOS) system in Arabidopsis thaliana cell culture under carbohydrate starvation supplied with a range of amino acids. Induction of isovaleryl-CoA dehydrogenase (IVDH) activity was observed under carbohydrate starvation which was associated with increased amounts of IVDH protein detected by immunoblotting. Furthermore, activities of the protein complexes of the mETC were reduced under carbohydrate starvation. We also observed that OXPHOS system activity behavior is differently affected by different amino acids and that proteins associated with amino acids catabolism are upregulated in cells following carbohydrate starvation. Collectively, our results support the contention that ETF/ETFQO is an essential pathway to donate electrons to the mETC and that amino acids are alternative substrates to maintain respiration under carbohydrate starvation.

AB - Plant respiration mostly depends on the activity of glycolysis and the oxidation of organic acids in the tricarboxylic acid cycle to synthesize ATP. However, during stress situations plant cells also use amino acids as alternative substrates to donate electrons through the electron-transfer flavoprotein (ETF)/ETF:ubiquinone oxidoreductase (ETF/ETFQO) complex to the mitochondrial electron transport chain (mETC). Given this, we investigated changes of the oxidative phosphorylation (OXPHOS) system in Arabidopsis thaliana cell culture under carbohydrate starvation supplied with a range of amino acids. Induction of isovaleryl-CoA dehydrogenase (IVDH) activity was observed under carbohydrate starvation which was associated with increased amounts of IVDH protein detected by immunoblotting. Furthermore, activities of the protein complexes of the mETC were reduced under carbohydrate starvation. We also observed that OXPHOS system activity behavior is differently affected by different amino acids and that proteins associated with amino acids catabolism are upregulated in cells following carbohydrate starvation. Collectively, our results support the contention that ETF/ETFQO is an essential pathway to donate electrons to the mETC and that amino acids are alternative substrates to maintain respiration under carbohydrate starvation.

UR - http://www.scopus.com/inward/record.url?scp=85034659893&partnerID=8YFLogxK

U2 - 10.1111/ppl.12612

DO - 10.1111/ppl.12612

M3 - Article

C2 - 28767134

AN - SCOPUS:85034659893

VL - 161

SP - 451

EP - 467

JO - Physiologia plantarum

JF - Physiologia plantarum

SN - 0031-9317

IS - 4

ER -