Detection of feruloyl- and cinnamoyl esterases from basidiomycetes in the presence of interfering laccase

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

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  • Paul Haase-Aschoff
  • Diana Linke
  • Ralf G. Berger

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OriginalspracheEnglisch
Seiten (von - bis)231-238
Seitenumfang8
FachzeitschriftBioresource technology
Jahrgang130
PublikationsstatusVeröffentlicht - 20 Dez. 2012

Abstract

Little is known on basidiomycete sources of feruloyl esterases (FAEs), although many wood-rotting representatives of these fungi typically grow on feruloyl-rich substrates. A major reason is that the almost ubiquitous presence of laccases interferes with the detection of FAE activity. Laccases polymerize the liberated ferulic acid (FA). in situ, thus detracting the product of enzymatic hydrolysis from its detection. A rapid HPLC-UV method was developed to detect the loss of FA, but also to quantify the hydrolysis of FA esters. The method allows at the same time to evaluate the substrate specificity of a FAE. Forty one basidiomycetes were tested for their FAE activities, and 25 out of the set were positive. The basidiomycetes hydrolyzing cinnamates with the highest conversion rates were. Auricularia auricula-judae and. Marasmius scorodonius. Moreover, a new FAE inducer, the nonionic detergent Tween 80, was found. This is the first comprehensive study on basidiomycete sources of FAEs.

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Detection of feruloyl- and cinnamoyl esterases from basidiomycetes in the presence of interfering laccase. / Haase-Aschoff, Paul; Linke, Diana; Berger, Ralf G.
in: Bioresource technology, Jahrgang 130, 20.12.2012, S. 231-238.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Haase-Aschoff P, Linke D, Berger RG. Detection of feruloyl- and cinnamoyl esterases from basidiomycetes in the presence of interfering laccase. Bioresource technology. 2012 Dez 20;130:231-238. doi: 10.1016/j.biortech.2012.12.039
Haase-Aschoff, Paul ; Linke, Diana ; Berger, Ralf G. / Detection of feruloyl- and cinnamoyl esterases from basidiomycetes in the presence of interfering laccase. in: Bioresource technology. 2012 ; Jahrgang 130. S. 231-238.
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abstract = "Little is known on basidiomycete sources of feruloyl esterases (FAEs), although many wood-rotting representatives of these fungi typically grow on feruloyl-rich substrates. A major reason is that the almost ubiquitous presence of laccases interferes with the detection of FAE activity. Laccases polymerize the liberated ferulic acid (FA). in situ, thus detracting the product of enzymatic hydrolysis from its detection. A rapid HPLC-UV method was developed to detect the loss of FA, but also to quantify the hydrolysis of FA esters. The method allows at the same time to evaluate the substrate specificity of a FAE. Forty one basidiomycetes were tested for their FAE activities, and 25 out of the set were positive. The basidiomycetes hydrolyzing cinnamates with the highest conversion rates were. Auricularia auricula-judae and. Marasmius scorodonius. Moreover, a new FAE inducer, the nonionic detergent Tween 80, was found. This is the first comprehensive study on basidiomycete sources of FAEs.",
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AU - Haase-Aschoff, Paul

AU - Linke, Diana

AU - Berger, Ralf G.

N1 - Funding information: Support of the work by the BMBF cluster Biokatalyse2021 (P 37) and by L. Popper (Sternenzym Ahrensburg, Germany) is gratefully acknowledged.

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N2 - Little is known on basidiomycete sources of feruloyl esterases (FAEs), although many wood-rotting representatives of these fungi typically grow on feruloyl-rich substrates. A major reason is that the almost ubiquitous presence of laccases interferes with the detection of FAE activity. Laccases polymerize the liberated ferulic acid (FA). in situ, thus detracting the product of enzymatic hydrolysis from its detection. A rapid HPLC-UV method was developed to detect the loss of FA, but also to quantify the hydrolysis of FA esters. The method allows at the same time to evaluate the substrate specificity of a FAE. Forty one basidiomycetes were tested for their FAE activities, and 25 out of the set were positive. The basidiomycetes hydrolyzing cinnamates with the highest conversion rates were. Auricularia auricula-judae and. Marasmius scorodonius. Moreover, a new FAE inducer, the nonionic detergent Tween 80, was found. This is the first comprehensive study on basidiomycete sources of FAEs.

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KW - Biofuels

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