Dengue-specific subviral nanoparticles: Design, creation and characterization

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autoren

  • Niyati Khetarpal
  • Ankur Poddar
  • Satish K. Nemani
  • Nisha Dhar
  • Aravind Patil
  • Priyanka Negi
  • Ashiya Perween
  • Ramaswamy Viswanathan
  • Heinrich Lünsdorf
  • Poornima Tyagi
  • Rajendra Raut
  • Upasana Arora
  • Swatantra K. Jain
  • Ursula Rinas
  • Sathyamangalam Swaminathan
  • Navin Khanna

Organisationseinheiten

Externe Organisationen

  • International Centre for Genetic Engineering and Biotechnology
  • Helmholtz-Zentrum für Infektionsforschung GmbH (HZI)
  • Jamia Hamdard
Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Aufsatznummer15
FachzeitschriftJournal of nanobiotechnology
Jahrgang11
Ausgabenummer1
PublikationsstatusVeröffentlicht - 25 Mai 2013

Abstract

Background: Dengue is today the most significant of arboviral diseases. Novel tools are necessary to effectively address the problem of dengue. Virus-like particles (VLP) offer a versatile nanoscale platform for developing tools with potential biomedical applications. From the perspective of a potentially useful dengue-specific tool, the dengue virus envelope protein domain III (EDIII), endowed with serotype-specificity, host receptor recognition and the capacity to elicit virus-neutralizing antibodies, is an attractive candidate.Methods: We have developed a strategy to co-express and co-purify Hepatitis B virus surface (S) antigen in two forms: independently and as a fusion with EDIII. We characterized these physically and functionally.Results: The two forms of the S antigen associate into VLPs. The ability of these to display EDIII in a functionally accessible manner is dependent upon the relative levels of the two forms of the S antigen. Mosaic VLPs containing the fused and un-fused components in 1:4 ratio displayed maximal functional competence.Conclusions: VLPs armed with EDIII may be potentially useful in diagnostic, therapeutic and prophylactic applications.

ASJC Scopus Sachgebiete

Ziele für nachhaltige Entwicklung

Zitieren

Dengue-specific subviral nanoparticles: Design, creation and characterization. / Khetarpal, Niyati; Poddar, Ankur; Nemani, Satish K. et al.
in: Journal of nanobiotechnology, Jahrgang 11, Nr. 1, 15, 25.05.2013.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Khetarpal, N, Poddar, A, Nemani, SK, Dhar, N, Patil, A, Negi, P, Perween, A, Viswanathan, R, Lünsdorf, H, Tyagi, P, Raut, R, Arora, U, Jain, SK, Rinas, U, Swaminathan, S & Khanna, N 2013, 'Dengue-specific subviral nanoparticles: Design, creation and characterization', Journal of nanobiotechnology, Jg. 11, Nr. 1, 15. https://doi.org/10.1186/1477-3155-11-15
Khetarpal, N., Poddar, A., Nemani, S. K., Dhar, N., Patil, A., Negi, P., Perween, A., Viswanathan, R., Lünsdorf, H., Tyagi, P., Raut, R., Arora, U., Jain, S. K., Rinas, U., Swaminathan, S., & Khanna, N. (2013). Dengue-specific subviral nanoparticles: Design, creation and characterization. Journal of nanobiotechnology, 11(1), Artikel 15. https://doi.org/10.1186/1477-3155-11-15
Khetarpal N, Poddar A, Nemani SK, Dhar N, Patil A, Negi P et al. Dengue-specific subviral nanoparticles: Design, creation and characterization. Journal of nanobiotechnology. 2013 Mai 25;11(1):15. doi: 10.1186/1477-3155-11-15
Khetarpal, Niyati ; Poddar, Ankur ; Nemani, Satish K. et al. / Dengue-specific subviral nanoparticles : Design, creation and characterization. in: Journal of nanobiotechnology. 2013 ; Jahrgang 11, Nr. 1.
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title = "Dengue-specific subviral nanoparticles: Design, creation and characterization",
abstract = "Background: Dengue is today the most significant of arboviral diseases. Novel tools are necessary to effectively address the problem of dengue. Virus-like particles (VLP) offer a versatile nanoscale platform for developing tools with potential biomedical applications. From the perspective of a potentially useful dengue-specific tool, the dengue virus envelope protein domain III (EDIII), endowed with serotype-specificity, host receptor recognition and the capacity to elicit virus-neutralizing antibodies, is an attractive candidate.Methods: We have developed a strategy to co-express and co-purify Hepatitis B virus surface (S) antigen in two forms: independently and as a fusion with EDIII. We characterized these physically and functionally.Results: The two forms of the S antigen associate into VLPs. The ability of these to display EDIII in a functionally accessible manner is dependent upon the relative levels of the two forms of the S antigen. Mosaic VLPs containing the fused and un-fused components in 1:4 ratio displayed maximal functional competence.Conclusions: VLPs armed with EDIII may be potentially useful in diagnostic, therapeutic and prophylactic applications.",
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note = "Funding Information: NiK and AnP are recipients of junior research fellowships from the Council of Scientific & Industrial Research, Government of India. SS and NaK are grateful to the Department of Biotechnology, Government of India for providing funds to perform this work. SS and NaK thank Drs. Harold Margolis, Dennis Trent, George Siber and Cristina Cassetti for their inputs in designing the mosaic VLPs.",
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TY - JOUR

T1 - Dengue-specific subviral nanoparticles

T2 - Design, creation and characterization

AU - Khetarpal, Niyati

AU - Poddar, Ankur

AU - Nemani, Satish K.

AU - Dhar, Nisha

AU - Patil, Aravind

AU - Negi, Priyanka

AU - Perween, Ashiya

AU - Viswanathan, Ramaswamy

AU - Lünsdorf, Heinrich

AU - Tyagi, Poornima

AU - Raut, Rajendra

AU - Arora, Upasana

AU - Jain, Swatantra K.

AU - Rinas, Ursula

AU - Swaminathan, Sathyamangalam

AU - Khanna, Navin

N1 - Funding Information: NiK and AnP are recipients of junior research fellowships from the Council of Scientific & Industrial Research, Government of India. SS and NaK are grateful to the Department of Biotechnology, Government of India for providing funds to perform this work. SS and NaK thank Drs. Harold Margolis, Dennis Trent, George Siber and Cristina Cassetti for their inputs in designing the mosaic VLPs.

PY - 2013/5/25

Y1 - 2013/5/25

N2 - Background: Dengue is today the most significant of arboviral diseases. Novel tools are necessary to effectively address the problem of dengue. Virus-like particles (VLP) offer a versatile nanoscale platform for developing tools with potential biomedical applications. From the perspective of a potentially useful dengue-specific tool, the dengue virus envelope protein domain III (EDIII), endowed with serotype-specificity, host receptor recognition and the capacity to elicit virus-neutralizing antibodies, is an attractive candidate.Methods: We have developed a strategy to co-express and co-purify Hepatitis B virus surface (S) antigen in two forms: independently and as a fusion with EDIII. We characterized these physically and functionally.Results: The two forms of the S antigen associate into VLPs. The ability of these to display EDIII in a functionally accessible manner is dependent upon the relative levels of the two forms of the S antigen. Mosaic VLPs containing the fused and un-fused components in 1:4 ratio displayed maximal functional competence.Conclusions: VLPs armed with EDIII may be potentially useful in diagnostic, therapeutic and prophylactic applications.

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KW - Dengue envelope domain III

KW - Hepatitis B surface antigen

KW - Pichia pastoris

KW - Virus-like particle

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DO - 10.1186/1477-3155-11-15

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JO - Journal of nanobiotechnology

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