Consensus statement on Brillouin light scattering microscopy of biological materials

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autorschaft

  • Pierre Bouvet
  • Carlo Bevilacqua
  • Yogeshwari Ambekar
  • Giuseppe Antonacci
  • Joshua Au
  • Silvia Caponi
  • Sophie Chagnon-Lessard
  • Jürgen Czarske
  • Thomas Dehoux
  • Daniele Fioretto
  • Yujian Fu
  • Jochen Guck
  • Thorsten Hamann
  • Dag Heinemann
  • Thorsten Jähnke
  • Hubert Jean-Ruel
  • Irina Kabakova
  • Kristie Koski
  • Nektarios Koukourakis
  • David Krause
  • Salvatore La Cavera III
  • Timm Landes
  • Jinhao Li
  • Hadi Mahmodi
  • Jeremie Margueritat
  • Maurizio Mattarelli
  • Michael Monaghan
  • Darryl R. Overby
  • Fernando Perez-Cota
  • Emanuele Pontecorvo
  • Robert Prevedel
  • Giancarlo Ruocco
  • John Sandercock
  • Giuliano Scarcelli
  • Filippo Scarponi
  • Claudia Testi
  • Peter Török
  • Lucie Vovard
  • Wolfgang J. Weninger
  • Vladislav Yakovlev
  • Seok-Hyun Yun
  • Jitao Zhang
  • Francesca Palombo
  • Alberto Bilenca
  • Kareem Elsayad

Externe Organisationen

  • Medizinische Universität Wien
  • European Molecular Biology Laboratory (EMBL)
  • University of Maryland
  • Specto Srl
  • CNR - Istituto Officina dei Materiali (IOM)
  • LightMachinery Inc.
  • Carleton University
  • Technische Universität Dresden (TUD)
  • Exzellenzcluster Physics of Life
  • Université Claude Bernard Lyon 1
  • Università degli Studi di Perugia
  • Max-Planck-Institut für die Physik des Lichts
  • Norwegian University of Science and Technology (NTNU)
  • CellSense Technologies GmbH
  • University of Technology Sydney
  • University of California at Davis
  • University of Nottingham
  • Trinity College Dublin
  • Imperial College London
  • CrestOptics S.p.A.
  • Deutsches Zentrum für Lungenforschung (DZL)
  • IIT - Center for Life Nano Science
  • The Table Stable Ltd.
  • Nanyang Technological University (NTU)
  • Texas A and M University
  • Harvard Medical School (HMS)
  • Wayne State University
  • University of Exeter
  • Ben-Gurion University of the Negev (BGU)
Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Seiten (von - bis)681–691
Seitenumfang11
FachzeitschriftNature Photonics
Jahrgang19
Ausgabenummer7
Frühes Online-Datum3 Juli 2025
PublikationsstatusVeröffentlicht - Juli 2025

Abstract

Brillouin light scattering (BLS) spectroscopy is a non-invasive, non-contact, label-free optical technique that can provide information on the mechanical properties of a material on the submicrometre scale. Over the past decade, BLS has found increasing microscopy applications in the life sciences, driven by the observed importance of mechanical properties in biological processes, the realization of more sensitive BLS spectrometers and the extension of BLS to an imaging modality. As with other spectroscopic techniques, BLS measurements detect not only signals that are characteristic of the investigated sample, but also those of the experimental apparatus, and can be substantially affected by measurement conditions. Here we report a consensus between researchers in the field. We aim to improve the comparability of BLS studies by providing reporting recommendations for the measured parameters and detailing common artefacts. Given that most BLS studies of biological matter are still at proof-of-concept stages and use different, often self-built, spectrometers, a consensus statement is particularly timely to ensure unified advancement.

ASJC Scopus Sachgebiete

Zitieren

Consensus statement on Brillouin light scattering microscopy of biological materials. / Bouvet, Pierre; Bevilacqua, Carlo; Ambekar, Yogeshwari et al.
in: Nature Photonics, Jahrgang 19, Nr. 7, 07.2025, S. 681–691.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Bouvet, P, Bevilacqua, C, Ambekar, Y, Antonacci, G, Au, J, Caponi, S, Chagnon-Lessard, S, Czarske, J, Dehoux, T, Fioretto, D, Fu, Y, Guck, J, Hamann, T, Heinemann, D, Jähnke, T, Jean-Ruel, H, Kabakova, I, Koski, K, Koukourakis, N, Krause, D, La Cavera III, S, Landes, T, Li, J, Mahmodi, H, Margueritat, J, Mattarelli, M, Monaghan, M, Overby, DR, Perez-Cota, F, Pontecorvo, E, Prevedel, R, Ruocco, G, Sandercock, J, Scarcelli, G, Scarponi, F, Testi, C, Török, P, Vovard, L, Weninger, WJ, Yakovlev, V, Yun, S-H, Zhang, J, Palombo, F, Bilenca, A & Elsayad, K 2025, 'Consensus statement on Brillouin light scattering microscopy of biological materials', Nature Photonics, Jg. 19, Nr. 7, S. 681–691. https://doi.org/10.1038/s41566-025-01681-6
Bouvet, P., Bevilacqua, C., Ambekar, Y., Antonacci, G., Au, J., Caponi, S., Chagnon-Lessard, S., Czarske, J., Dehoux, T., Fioretto, D., Fu, Y., Guck, J., Hamann, T., Heinemann, D., Jähnke, T., Jean-Ruel, H., Kabakova, I., Koski, K., Koukourakis, N., ... Elsayad, K. (2025). Consensus statement on Brillouin light scattering microscopy of biological materials. Nature Photonics, 19(7), 681–691. https://doi.org/10.1038/s41566-025-01681-6
Bouvet P, Bevilacqua C, Ambekar Y, Antonacci G, Au J, Caponi S et al. Consensus statement on Brillouin light scattering microscopy of biological materials. Nature Photonics. 2025 Jul;19(7):681–691. Epub 2025 Jul 3. doi: 10.1038/s41566-025-01681-6
Bouvet, Pierre ; Bevilacqua, Carlo ; Ambekar, Yogeshwari et al. / Consensus statement on Brillouin light scattering microscopy of biological materials. in: Nature Photonics. 2025 ; Jahrgang 19, Nr. 7. S. 681–691.
Download
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title = "Consensus statement on Brillouin light scattering microscopy of biological materials",
abstract = "Brillouin light scattering (BLS) spectroscopy is a non-invasive, non-contact, label-free optical technique that can provide information on the mechanical properties of a material on the submicrometre scale. Over the past decade, BLS has found increasing microscopy applications in the life sciences, driven by the observed importance of mechanical properties in biological processes, the realization of more sensitive BLS spectrometers and the extension of BLS to an imaging modality. As with other spectroscopic techniques, BLS measurements detect not only signals that are characteristic of the investigated sample, but also those of the experimental apparatus, and can be substantially affected by measurement conditions. Here we report a consensus between researchers in the field. We aim to improve the comparability of BLS studies by providing reporting recommendations for the measured parameters and detailing common artefacts. Given that most BLS studies of biological matter are still at proof-of-concept stages and use different, often self-built, spectrometers, a consensus statement is particularly timely to ensure unified advancement.",
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AU - Bouvet, Pierre

AU - Bevilacqua, Carlo

AU - Ambekar, Yogeshwari

AU - Antonacci, Giuseppe

AU - Au, Joshua

AU - Caponi, Silvia

AU - Chagnon-Lessard, Sophie

AU - Czarske, Jürgen

AU - Dehoux, Thomas

AU - Fioretto, Daniele

AU - Fu, Yujian

AU - Guck, Jochen

AU - Hamann, Thorsten

AU - Heinemann, Dag

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AU - Koski, Kristie

AU - Koukourakis, Nektarios

AU - Krause, David

AU - La Cavera III, Salvatore

AU - Landes, Timm

AU - Li, Jinhao

AU - Mahmodi, Hadi

AU - Margueritat, Jeremie

AU - Mattarelli, Maurizio

AU - Monaghan, Michael

AU - Overby, Darryl R.

AU - Perez-Cota, Fernando

AU - Pontecorvo, Emanuele

AU - Prevedel, Robert

AU - Ruocco, Giancarlo

AU - Sandercock, John

AU - Scarcelli, Giuliano

AU - Scarponi, Filippo

AU - Testi, Claudia

AU - Török, Peter

AU - Vovard, Lucie

AU - Weninger, Wolfgang J.

AU - Yakovlev, Vladislav

AU - Yun, Seok-Hyun

AU - Zhang, Jitao

AU - Palombo, Francesca

AU - Bilenca, Alberto

AU - Elsayad, Kareem

N1 - Publisher Copyright: © The Author(s), under exclusive licence to Springer Nature Limited 2025.

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