Chemoenzymatic Synthesis of Structurally Diverse Terpenoids from Farnesyl Pyrophosphates Modified at the Central Alkene Unit

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

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  • Technische Universität Darmstadt
  • Uppsala University
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Details

OriginalspracheEnglisch
Seiten (von - bis)525-536
Seitenumfang12
FachzeitschriftJournal of the American Chemical Society
Jahrgang148
Ausgabenummer1
Frühes Online-Datum22 Dez. 2025
PublikationsstatusVeröffentlicht - 14 Jan. 2026

Abstract

Sesquiterpene synthases (STSs) enable cationic cascade reactions with farnesyl pyrophosphate (FPP) resulting in an immense variety of oligocyclic sesquiterpenes. Their substrate promiscuity allows access to new sesquiterpene carbon skeletons. We explored the ability of eight STSs to process three distinct synthetic FPP derivatives modified at the central isoprenyl unit. These include the incorporation of a keto group at C7 (“keto”-FPP), the relocation of the olefinic double bond into the methyl group (“iso”-FPP), and the shift of the double bond toward the aliphatic terminus of the FPP backbone and loss of the methyl group at C7 (“nor-iso” FPP). We report the enzymatic production of 18 new terpenoids, including a large variety of new oxaterpenoids. One of these is known as a late stage intermediate in the total synthesis of the sex pheromone periplanone B, which is secreted by females of the American cockroach Periplaneta americana to attract mates. Thus, a formal chemoenzymatic synthesis of this pheromone is disclosed.

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Chemoenzymatic Synthesis of Structurally Diverse Terpenoids from Farnesyl Pyrophosphates Modified at the Central Alkene Unit. / Struwe, Henry; Slotman, Christopher; Höft, Laurent et al.
in: Journal of the American Chemical Society, Jahrgang 148, Nr. 1, 14.01.2026, S. 525-536.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Struwe H, Slotman C, Höft L, Dräger G, Droste J, Fohrer J et al. Chemoenzymatic Synthesis of Structurally Diverse Terpenoids from Farnesyl Pyrophosphates Modified at the Central Alkene Unit. Journal of the American Chemical Society. 2026 Jan 14;148(1):525-536. Epub 2025 Dez 22. doi: 10.1021/jacs.5c14636
Struwe, Henry ; Slotman, Christopher ; Höft, Laurent et al. / Chemoenzymatic Synthesis of Structurally Diverse Terpenoids from Farnesyl Pyrophosphates Modified at the Central Alkene Unit. in: Journal of the American Chemical Society. 2026 ; Jahrgang 148, Nr. 1. S. 525-536.
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AU - Beutel, Sascha

AU - Kolling, Dominik

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