Bioavailability of rosehip (Rosa canina L.) infusion phenolics prepared by thermal, pulsed electric field and high pressure processing

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OriginalspracheEnglisch
Aufsatznummer1955
FachzeitschriftFoods
Jahrgang11
Ausgabenummer13
PublikationsstatusVeröffentlicht - 1 Juli 2022

Abstract

In this study, the in vitro bioavailability of rosehip infusion phenolics, mainly catechin, as a response to conventional and non-thermal treatments by combining gastrointestinal digestion and a Caco-2 cell culture model, was investigated. After application of thermal treatment (TT, 85 C/10 min), high pressure (HPP, 600 MPa/5 min) or pulsed electric field (PEF, 15 kJ/kg) processing, all samples were subjected to simulated gastrointestinal digestion. Then, the amount of maximum non-toxic digest ratio was determined by the cytotoxicity sulforhodamine B (SRB) assay. Next, Caco-2 cells were exposed to 1:5 (v/v) times diluted digests in order to simulate the transepithelial transportation of catechin. Results showed that non-thermally processed samples (5.19 and 4.62% for HPP and PEF, respectively) exhibited greater transportation across the epithelial cell layer compared to than that of the TT-treated sample (3.42%). The present study highlighted that HPP and PEF, as non-thermal treatments at optimized conditions for infusions or beverages, can be utilized in order to enhance the nutritional quality of the final products.

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Bioavailability of rosehip (Rosa canina L.) infusion phenolics prepared by thermal, pulsed electric field and high pressure processing. / Ozkan, Gulay; Esatbeyoglu, Tuba; Capanoglu, Esra.
in: Foods, Jahrgang 11, Nr. 13, 1955, 01.07.2022.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

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abstract = "In this study, the in vitro bioavailability of rosehip infusion phenolics, mainly catechin, as a response to conventional and non-thermal treatments by combining gastrointestinal digestion and a Caco-2 cell culture model, was investigated. After application of thermal treatment (TT, 85 ◦C/10 min), high pressure (HPP, 600 MPa/5 min) or pulsed electric field (PEF, 15 kJ/kg) processing, all samples were subjected to simulated gastrointestinal digestion. Then, the amount of maximum non-toxic digest ratio was determined by the cytotoxicity sulforhodamine B (SRB) assay. Next, Caco-2 cells were exposed to 1:5 (v/v) times diluted digests in order to simulate the transepithelial transportation of catechin. Results showed that non-thermally processed samples (5.19 and 4.62% for HPP and PEF, respectively) exhibited greater transportation across the epithelial cell layer compared to than that of the TT-treated sample (3.42%). The present study highlighted that HPP and PEF, as non-thermal treatments at optimized conditions for infusions or beverages, can be utilized in order to enhance the nutritional quality of the final products. ",
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author = "Gulay Ozkan and Tuba Esatbeyoglu and Esra Capanoglu",
note = "Funding Information: The publication of this article was funded by the Open Access Fund of Leibniz Universit{\"a}t Hannover. The authors would like to acknowledge Gerald Dr{\"a}ger for his guidance during LC-MS analysis and Kemal Aganovic from the German Institute of Food Technologies DIL for providing the HPP and PEF devices.",
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T1 - Bioavailability of rosehip (Rosa canina L.) infusion phenolics prepared by thermal, pulsed electric field and high pressure processing

AU - Ozkan, Gulay

AU - Esatbeyoglu, Tuba

AU - Capanoglu, Esra

N1 - Funding Information: The publication of this article was funded by the Open Access Fund of Leibniz Universität Hannover. The authors would like to acknowledge Gerald Dräger for his guidance during LC-MS analysis and Kemal Aganovic from the German Institute of Food Technologies DIL for providing the HPP and PEF devices.

PY - 2022/7/1

Y1 - 2022/7/1

N2 - In this study, the in vitro bioavailability of rosehip infusion phenolics, mainly catechin, as a response to conventional and non-thermal treatments by combining gastrointestinal digestion and a Caco-2 cell culture model, was investigated. After application of thermal treatment (TT, 85 ◦C/10 min), high pressure (HPP, 600 MPa/5 min) or pulsed electric field (PEF, 15 kJ/kg) processing, all samples were subjected to simulated gastrointestinal digestion. Then, the amount of maximum non-toxic digest ratio was determined by the cytotoxicity sulforhodamine B (SRB) assay. Next, Caco-2 cells were exposed to 1:5 (v/v) times diluted digests in order to simulate the transepithelial transportation of catechin. Results showed that non-thermally processed samples (5.19 and 4.62% for HPP and PEF, respectively) exhibited greater transportation across the epithelial cell layer compared to than that of the TT-treated sample (3.42%). The present study highlighted that HPP and PEF, as non-thermal treatments at optimized conditions for infusions or beverages, can be utilized in order to enhance the nutritional quality of the final products.

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