Details
| Originalsprache | Englisch |
|---|---|
| Aufsatznummer | 1591861 |
| Fachzeitschrift | Frontiers in Plant Science |
| Jahrgang | 16 |
| Publikationsstatus | Veröffentlicht - 13 Mai 2025 |
Abstract
Introduction: Breeding roses with ideal ornamental characteristics such as beautiful flowers, a pleasant fragrance, and attractive growth habits is complex and time-consuming. This process can be improved and accelerated through the use of molecular markers. Methods: We conducted a genome-wide association study on nine ornamental traits in roses using the RhWagSNP chip across a panel of 285 cultivars and varieties. Significant marker-trait associations found for major quantitative trait loci were further validated using single-marker analyses with PACE technology in independent panels of up to 182 genotypes. Results: For six traits— ‘Young shoot: intensity of anthocyanin coloration’, ‘Stem: number of prickles’, ‘Leaf: glossiness of upper side’, ‘Flower: number of petals’, ‘Flower: fragrance’, and ‘Petal: length’—we identified and validated marker-trait associations for major QTLs. Conversely, we were unable to validate associations for ‘Leaf: anthocyanin coloration’ and ‘Leaf: intensity of green color on the upper side’, and found no significant associations in the GWAS for ‘Leaf: size’. Loci that affect petal size, petal number and fragrance have been previously studied. We were able to detect associated markers with increased effect sizes for all three traits. Even greater effects were observed when we combined markers from independent loci for petal number and fragrance. Discussion: Associated markers for some of the analysed traits largely colocalise with markers previously identified in QTL analyses of biparental populations. Our validation strategy using PACE as an alternative marker technology in independent panels and different environments supports the robustness of our data, irrespective of our limited panel sizes. For the six traits for which we could validate marker-trait associations, our data can be interpreted cautiously as indicating high complexity of inheritance, with few large-effect QTLs influencing the traits. For the other four traits, either greater genetic complexity and/or stronger environmental effects may have confounded our analyses. We believe that the markers presented here can serve as valuable tools for marker-assisted selection and for further genetic analysis of the traits we have analysed.
ASJC Scopus Sachgebiete
- Agrar- und Biowissenschaften (insg.)
- Pflanzenkunde
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in: Frontiers in Plant Science, Jahrgang 16, 1591861, 13.05.2025.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Beyond bloom
T2 - validated marker–trait discovery for polyploid roses via GWAS
AU - Patzer, Laurine
AU - Schulz, Dietmar Frank
AU - Ezeoke, Amarachi Queendaline
AU - Linde, Marcus
AU - Debener, Thomas
N1 - Publisher Copyright: Copyright © 2025 Patzer, Schulz, Ezeoke, Linde and Debener.
PY - 2025/5/13
Y1 - 2025/5/13
N2 - Introduction: Breeding roses with ideal ornamental characteristics such as beautiful flowers, a pleasant fragrance, and attractive growth habits is complex and time-consuming. This process can be improved and accelerated through the use of molecular markers. Methods: We conducted a genome-wide association study on nine ornamental traits in roses using the RhWagSNP chip across a panel of 285 cultivars and varieties. Significant marker-trait associations found for major quantitative trait loci were further validated using single-marker analyses with PACE technology in independent panels of up to 182 genotypes. Results: For six traits— ‘Young shoot: intensity of anthocyanin coloration’, ‘Stem: number of prickles’, ‘Leaf: glossiness of upper side’, ‘Flower: number of petals’, ‘Flower: fragrance’, and ‘Petal: length’—we identified and validated marker-trait associations for major QTLs. Conversely, we were unable to validate associations for ‘Leaf: anthocyanin coloration’ and ‘Leaf: intensity of green color on the upper side’, and found no significant associations in the GWAS for ‘Leaf: size’. Loci that affect petal size, petal number and fragrance have been previously studied. We were able to detect associated markers with increased effect sizes for all three traits. Even greater effects were observed when we combined markers from independent loci for petal number and fragrance. Discussion: Associated markers for some of the analysed traits largely colocalise with markers previously identified in QTL analyses of biparental populations. Our validation strategy using PACE as an alternative marker technology in independent panels and different environments supports the robustness of our data, irrespective of our limited panel sizes. For the six traits for which we could validate marker-trait associations, our data can be interpreted cautiously as indicating high complexity of inheritance, with few large-effect QTLs influencing the traits. For the other four traits, either greater genetic complexity and/or stronger environmental effects may have confounded our analyses. We believe that the markers presented here can serve as valuable tools for marker-assisted selection and for further genetic analysis of the traits we have analysed.
AB - Introduction: Breeding roses with ideal ornamental characteristics such as beautiful flowers, a pleasant fragrance, and attractive growth habits is complex and time-consuming. This process can be improved and accelerated through the use of molecular markers. Methods: We conducted a genome-wide association study on nine ornamental traits in roses using the RhWagSNP chip across a panel of 285 cultivars and varieties. Significant marker-trait associations found for major quantitative trait loci were further validated using single-marker analyses with PACE technology in independent panels of up to 182 genotypes. Results: For six traits— ‘Young shoot: intensity of anthocyanin coloration’, ‘Stem: number of prickles’, ‘Leaf: glossiness of upper side’, ‘Flower: number of petals’, ‘Flower: fragrance’, and ‘Petal: length’—we identified and validated marker-trait associations for major QTLs. Conversely, we were unable to validate associations for ‘Leaf: anthocyanin coloration’ and ‘Leaf: intensity of green color on the upper side’, and found no significant associations in the GWAS for ‘Leaf: size’. Loci that affect petal size, petal number and fragrance have been previously studied. We were able to detect associated markers with increased effect sizes for all three traits. Even greater effects were observed when we combined markers from independent loci for petal number and fragrance. Discussion: Associated markers for some of the analysed traits largely colocalise with markers previously identified in QTL analyses of biparental populations. Our validation strategy using PACE as an alternative marker technology in independent panels and different environments supports the robustness of our data, irrespective of our limited panel sizes. For the six traits for which we could validate marker-trait associations, our data can be interpreted cautiously as indicating high complexity of inheritance, with few large-effect QTLs influencing the traits. For the other four traits, either greater genetic complexity and/or stronger environmental effects may have confounded our analyses. We believe that the markers presented here can serve as valuable tools for marker-assisted selection and for further genetic analysis of the traits we have analysed.
KW - breeding
KW - genome-wide association studies
KW - MAS
KW - molecular markers
KW - polyploid
KW - QTL
KW - rose ornamental traits
KW - SNP array
UR - http://www.scopus.com/inward/record.url?scp=105006681007&partnerID=8YFLogxK
U2 - 10.3389/fpls.2025.1591861
DO - 10.3389/fpls.2025.1591861
M3 - Article
AN - SCOPUS:105006681007
VL - 16
JO - Frontiers in Plant Science
JF - Frontiers in Plant Science
SN - 1664-462X
M1 - 1591861
ER -