Beet mosaic virus expression of a betalain transcription factor allows visual virus tracking in Beta vulgaris

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autoren

  • Lukas Rollwage
  • Edgar Maiss
  • Wulf Menzel
  • Roxana Hossain
  • Mark Varrelmann

Externe Organisationen

  • Institut für Zuckerrübenforschung (IfZ)
  • Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Seiten (von - bis)1319-1329
Seitenumfang11
FachzeitschriftMolecular plant pathology
Jahrgang24
Ausgabenummer10
PublikationsstatusVeröffentlicht - 15 Sept. 2023

Abstract

In the field of plant virology, the usage of reverse genetic systems has been reported for multiple purposes. One is understanding virus–host interaction by labelling viral cDNA clones with fluorescent protein genes to allow visual virus tracking throughout a plant, albeit this visualization depends on technical devices. Here we report the first construction of an infectious cDNA full-length clone of beet mosaic virus (BtMV) that can be efficiently used for Agrobacterium-mediated leaf inoculation with high infection rate in Beta vulgaris, being indistinguishable from the natural virus isolate regarding symptom development and vector transmission. Furthermore, the BtMV clone was tagged with the genes for the monomeric red fluorescent protein or the Beta vulgaris BvMYB1 transcription factor, which activates the betalain biosynthesis pathway. The heterologous expression of BvMYB1 results in activation of betalain biosynthesis genes in planta, allowing visualization of the systemic BtMV spread with the naked eye as red pigmentation emerging throughout beet leaves. In the case of BtMV, the BvMYB1 marker system is stable over multiple mechanical host passages, allows qualitative as well as quantitative virus detection and offers an excellent opportunity to label viruses in plants of the order Caryophyllales, allowing an in-depth investigation of virus–host interactions on the whole plant level.

ASJC Scopus Sachgebiete

Zitieren

Beet mosaic virus expression of a betalain transcription factor allows visual virus tracking in Beta vulgaris. / Rollwage, Lukas; Maiss, Edgar; Menzel, Wulf et al.
in: Molecular plant pathology, Jahrgang 24, Nr. 10, 15.09.2023, S. 1319-1329.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Rollwage L, Maiss E, Menzel W, Hossain R, Varrelmann M. Beet mosaic virus expression of a betalain transcription factor allows visual virus tracking in Beta vulgaris. Molecular plant pathology. 2023 Sep 15;24(10):1319-1329. doi: 10.1111/mpp.13372
Rollwage, Lukas ; Maiss, Edgar ; Menzel, Wulf et al. / Beet mosaic virus expression of a betalain transcription factor allows visual virus tracking in Beta vulgaris. in: Molecular plant pathology. 2023 ; Jahrgang 24, Nr. 10. S. 1319-1329.
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abstract = "In the field of plant virology, the usage of reverse genetic systems has been reported for multiple purposes. One is understanding virus–host interaction by labelling viral cDNA clones with fluorescent protein genes to allow visual virus tracking throughout a plant, albeit this visualization depends on technical devices. Here we report the first construction of an infectious cDNA full-length clone of beet mosaic virus (BtMV) that can be efficiently used for Agrobacterium-mediated leaf inoculation with high infection rate in Beta vulgaris, being indistinguishable from the natural virus isolate regarding symptom development and vector transmission. Furthermore, the BtMV clone was tagged with the genes for the monomeric red fluorescent protein or the Beta vulgaris BvMYB1 transcription factor, which activates the betalain biosynthesis pathway. The heterologous expression of BvMYB1 results in activation of betalain biosynthesis genes in planta, allowing visualization of the systemic BtMV spread with the naked eye as red pigmentation emerging throughout beet leaves. In the case of BtMV, the BvMYB1 marker system is stable over multiple mechanical host passages, allows qualitative as well as quantitative virus detection and offers an excellent opportunity to label viruses in plants of the order Caryophyllales, allowing an in-depth investigation of virus–host interactions on the whole plant level.",
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AU - Rollwage, Lukas

AU - Maiss, Edgar

AU - Menzel, Wulf

AU - Hossain, Roxana

AU - Varrelmann, Mark

N1 - Funding Information: The authors would like to thank all staff of the Institute of Sugar Beet Research, Leibniz University Hannover and Leibniz Institute DSMZ for their technical support on laboratory work.

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N2 - In the field of plant virology, the usage of reverse genetic systems has been reported for multiple purposes. One is understanding virus–host interaction by labelling viral cDNA clones with fluorescent protein genes to allow visual virus tracking throughout a plant, albeit this visualization depends on technical devices. Here we report the first construction of an infectious cDNA full-length clone of beet mosaic virus (BtMV) that can be efficiently used for Agrobacterium-mediated leaf inoculation with high infection rate in Beta vulgaris, being indistinguishable from the natural virus isolate regarding symptom development and vector transmission. Furthermore, the BtMV clone was tagged with the genes for the monomeric red fluorescent protein or the Beta vulgaris BvMYB1 transcription factor, which activates the betalain biosynthesis pathway. The heterologous expression of BvMYB1 results in activation of betalain biosynthesis genes in planta, allowing visualization of the systemic BtMV spread with the naked eye as red pigmentation emerging throughout beet leaves. In the case of BtMV, the BvMYB1 marker system is stable over multiple mechanical host passages, allows qualitative as well as quantitative virus detection and offers an excellent opportunity to label viruses in plants of the order Caryophyllales, allowing an in-depth investigation of virus–host interactions on the whole plant level.

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