Bacterial Diversity Controls Transformation of Wastewater-Derived Organic Contaminants in River-Simulating Flumes

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autoren

  • Malte Posselt
  • Jonas Mechelke
  • Cyrus Rutere
  • Claudia Coll
  • Anna Jaeger
  • Muhammad Raza
  • Karin Meinikmann
  • Stefan Krause
  • Anna Sobek
  • Jörg Lewandowski
  • Marcus A Horn
  • Juliane Hollender
  • Jonathan P Benskin

Organisationseinheiten

Externe Organisationen

  • Stockholm University
  • Swiss Federal Institute of Aquatic Science and Technology (Eawag)
  • Universität Bayreuth
  • Leibniz-Institut für Gewässerökologie und Binnenfischerei (IGB)
  • Technische Universität Darmstadt
  • University of Birmingham
Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Seiten (von - bis)5467-5479
Seitenumfang13
FachzeitschriftEnvironmental Science & Technology
Jahrgang54
Ausgabenummer9
Frühes Online-Datum6 Apr. 2020
PublikationsstatusVeröffentlicht - 5 Mai 2020

Abstract

Hyporheic zones are the water-saturated flow-through subsurfaces of rivers which are characterized by the simultaneous occurrence of multiple physical, biological, and chemical processes. Two factors playing a role in the hyporheic attenuation of organic contaminants are sediment bedforms (a major driver of hyporheic exchange) and the composition of the sediment microbial community. How these factors act on the diverse range of organic contaminants encountered downstream from wastewater treatment plants is not well understood. To address this knowledge gap, we investigated dissipation half-lives (DT50s) of 31 substances (mainly pharmaceuticals) under different combinations of bacterial diversity and bedform-induced hyporheic flow using 20 recirculating flumes in a central composite face factorial design. By combining small-volume pore water sampling, targeted analysis, and suspect screening, along with quantitative real-time PCR and time-resolved amplicon Illumina MiSeq sequencing, we determined a comprehensive set of DT50s, associated bacterial communities, and microbial transformation products. The resulting DT50s of parent compounds ranged from 0.5 (fluoxetine) to 306 days (carbamazepine), with 20 substances responding significantly to bacterial diversity and four to both diversity and hyporheic flow. Bacterial taxa that were associated with biodegradation included Acidobacteria (groups 6, 17, and 22), Actinobacteria (Nocardioides and Illumatobacter), Bacteroidetes (Terrimonas and Flavobacterium) and diverse Proteobacteria (Pseudomonadaceae, Sphingomonadaceae, and Xanthomonadaceae). Notable were the formation of valsartan acid from irbesartan and valsartan, the persistence of N-desmethylvenlafaxine across all treatments, and the identification of biuret as a novel transformation product of metformin. Twelve additional target transformation products were identified, which were persistent in either pore or surface water of at least one treatment, indicating their environmental relevance.

ASJC Scopus Sachgebiete

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Bacterial Diversity Controls Transformation of Wastewater-Derived Organic Contaminants in River-Simulating Flumes. / Posselt, Malte; Mechelke, Jonas; Rutere, Cyrus et al.
in: Environmental Science & Technology, Jahrgang 54, Nr. 9, 05.05.2020, S. 5467-5479.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Posselt, M, Mechelke, J, Rutere, C, Coll, C, Jaeger, A, Raza, M, Meinikmann, K, Krause, S, Sobek, A, Lewandowski, J, Horn, MA, Hollender, J & Benskin, JP 2020, 'Bacterial Diversity Controls Transformation of Wastewater-Derived Organic Contaminants in River-Simulating Flumes', Environmental Science & Technology, Jg. 54, Nr. 9, S. 5467-5479. https://doi.org/10.1021/acs.est.9b06928, https://doi.org/10.1021/acs.est.0c03798
Posselt, M., Mechelke, J., Rutere, C., Coll, C., Jaeger, A., Raza, M., Meinikmann, K., Krause, S., Sobek, A., Lewandowski, J., Horn, M. A., Hollender, J., & Benskin, J. P. (2020). Bacterial Diversity Controls Transformation of Wastewater-Derived Organic Contaminants in River-Simulating Flumes. Environmental Science & Technology, 54(9), 5467-5479. https://doi.org/10.1021/acs.est.9b06928, https://doi.org/10.1021/acs.est.0c03798
Posselt M, Mechelke J, Rutere C, Coll C, Jaeger A, Raza M et al. Bacterial Diversity Controls Transformation of Wastewater-Derived Organic Contaminants in River-Simulating Flumes. Environmental Science & Technology. 2020 Mai 5;54(9):5467-5479. Epub 2020 Apr 6. doi: 10.1021/acs.est.9b06928, 10.1021/acs.est.0c03798
Posselt, Malte ; Mechelke, Jonas ; Rutere, Cyrus et al. / Bacterial Diversity Controls Transformation of Wastewater-Derived Organic Contaminants in River-Simulating Flumes. in: Environmental Science & Technology. 2020 ; Jahrgang 54, Nr. 9. S. 5467-5479.
Download
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TY - JOUR

T1 - Bacterial Diversity Controls Transformation of Wastewater-Derived Organic Contaminants in River-Simulating Flumes

AU - Posselt, Malte

AU - Mechelke, Jonas

AU - Rutere, Cyrus

AU - Coll, Claudia

AU - Jaeger, Anna

AU - Raza, Muhammad

AU - Meinikmann, Karin

AU - Krause, Stefan

AU - Sobek, Anna

AU - Lewandowski, Jörg

AU - Horn, Marcus A

AU - Hollender, Juliane

AU - Benskin, Jonathan P

N1 - Funding Information: This project has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement No. 641939 and the Swiss State Secretariat for Education, Research and Innovation.

PY - 2020/5/5

Y1 - 2020/5/5

N2 - Hyporheic zones are the water-saturated flow-through subsurfaces of rivers which are characterized by the simultaneous occurrence of multiple physical, biological, and chemical processes. Two factors playing a role in the hyporheic attenuation of organic contaminants are sediment bedforms (a major driver of hyporheic exchange) and the composition of the sediment microbial community. How these factors act on the diverse range of organic contaminants encountered downstream from wastewater treatment plants is not well understood. To address this knowledge gap, we investigated dissipation half-lives (DT50s) of 31 substances (mainly pharmaceuticals) under different combinations of bacterial diversity and bedform-induced hyporheic flow using 20 recirculating flumes in a central composite face factorial design. By combining small-volume pore water sampling, targeted analysis, and suspect screening, along with quantitative real-time PCR and time-resolved amplicon Illumina MiSeq sequencing, we determined a comprehensive set of DT50s, associated bacterial communities, and microbial transformation products. The resulting DT50s of parent compounds ranged from 0.5 (fluoxetine) to 306 days (carbamazepine), with 20 substances responding significantly to bacterial diversity and four to both diversity and hyporheic flow. Bacterial taxa that were associated with biodegradation included Acidobacteria (groups 6, 17, and 22), Actinobacteria (Nocardioides and Illumatobacter), Bacteroidetes (Terrimonas and Flavobacterium) and diverse Proteobacteria (Pseudomonadaceae, Sphingomonadaceae, and Xanthomonadaceae). Notable were the formation of valsartan acid from irbesartan and valsartan, the persistence of N-desmethylvenlafaxine across all treatments, and the identification of biuret as a novel transformation product of metformin. Twelve additional target transformation products were identified, which were persistent in either pore or surface water of at least one treatment, indicating their environmental relevance.

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