Assessment of oxygen kinetic parameters for closely related ammonia-oxidizing bacteria

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autoren

  • Pallabita Saha
  • Ann Kathrin Kniggendorf
  • Andreas Pommerening-Röser
  • Regina Nogueira

Externe Organisationen

  • Physikalisch-Technische Bundesanstalt (PTB)
  • Universität Hamburg
Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Aufsatznummerovae076
FachzeitschriftLetters in applied microbiology
Jahrgang77
Ausgabenummer8
Frühes Online-Datum6 Aug. 2024
PublikationsstatusVeröffentlicht - Aug. 2024

Abstract

The reaction kinetics of lithotrophic ammonia-oxidizing bacteria (AOB) are strongly dependent on dissolved oxygen (DO) as their metabolism is an aerobic process. In this study, we estimate the kinetic parameters, including the oxygen affinity constant (Km[O2]) and the maximum oxygen consumption rate (Vmax[O2]), of different AOB species, by fitting the data to the Michaelis–Menten equation using nonlinear regression analysis. An example for three different species of Nitrosomonas bacteria (N. europaea, N. eutropha, and N. mobilis) in monoculture is given, finding a Km[O2] of 0.25 ± 0.05 mg l−1, 0.47 ± 0.09 mg l−1, and 0.28 ± 0.08 mg l−1, and a Vmax[O2] of 0.07 ± 0.04 pg h−1cell−1, 0.25 ± 0.06 pg h−1cell−1, and 0.02 ± 0.001 pg h−1cell−1 for N. europaea, N. eutropha, and N. mobilis, respectively. This study shows that of the analyzed AOB, N. europaea has the highest affinity towards oxygen and N. eutropha the lowest affinity towards oxygen, indicating that the former can convert ammonia even under low DO conditions. These results improve the understanding of the ecophysiology of AOB in the environment. The accuracy of mathematically modelled ammonia oxidation can be improved, allowing the implementation of better management practices to restore the nitrogen cycle in natural and engineered water systems.

ASJC Scopus Sachgebiete

Zitieren

Assessment of oxygen kinetic parameters for closely related ammonia-oxidizing bacteria. / Saha, Pallabita; Kniggendorf, Ann Kathrin; Pommerening-Röser, Andreas et al.
in: Letters in applied microbiology, Jahrgang 77, Nr. 8, ovae076, 08.2024.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Saha P, Kniggendorf AK, Pommerening-Röser A, Nogueira R. Assessment of oxygen kinetic parameters for closely related ammonia-oxidizing bacteria. Letters in applied microbiology. 2024 Aug;77(8):ovae076. Epub 2024 Aug 6. doi: 10.1093/lambio/ovae076
Saha, Pallabita ; Kniggendorf, Ann Kathrin ; Pommerening-Röser, Andreas et al. / Assessment of oxygen kinetic parameters for closely related ammonia-oxidizing bacteria. in: Letters in applied microbiology. 2024 ; Jahrgang 77, Nr. 8.
Download
@article{dd0f1a913d9c4dc2abaf33f5ba0cafc9,
title = "Assessment of oxygen kinetic parameters for closely related ammonia-oxidizing bacteria",
abstract = "The reaction kinetics of lithotrophic ammonia-oxidizing bacteria (AOB) are strongly dependent on dissolved oxygen (DO) as their metabolism is an aerobic process. In this study, we estimate the kinetic parameters, including the oxygen affinity constant (Km[O2]) and the maximum oxygen consumption rate (Vmax[O2]), of different AOB species, by fitting the data to the Michaelis–Menten equation using nonlinear regression analysis. An example for three different species of Nitrosomonas bacteria (N. europaea, N. eutropha, and N. mobilis) in monoculture is given, finding a Km[O2] of 0.25 ± 0.05 mg l−1, 0.47 ± 0.09 mg l−1, and 0.28 ± 0.08 mg l−1, and a Vmax[O2] of 0.07 ± 0.04 pg h−1cell−1, 0.25 ± 0.06 pg h−1cell−1, and 0.02 ± 0.001 pg h−1cell−1 for N. europaea, N. eutropha, and N. mobilis, respectively. This study shows that of the analyzed AOB, N. europaea has the highest affinity towards oxygen and N. eutropha the lowest affinity towards oxygen, indicating that the former can convert ammonia even under low DO conditions. These results improve the understanding of the ecophysiology of AOB in the environment. The accuracy of mathematically modelled ammonia oxidation can be improved, allowing the implementation of better management practices to restore the nitrogen cycle in natural and engineered water systems.",
keywords = "ammonia oxidizing bacteria (aob), kinetics, non-linear regression analysis, oxygen affinity constant, parameter estimation",
author = "Pallabita Saha and Kniggendorf, {Ann Kathrin} and Andreas Pommerening-R{\"o}ser and Regina Nogueira",
note = "Publisher Copyright: {\textcopyright} The Author(s) 2024. Published by Oxford University Press on behalf of Applied Microbiology International. All rights reserved.",
year = "2024",
month = aug,
doi = "10.1093/lambio/ovae076",
language = "English",
volume = "77",
journal = "Letters in applied microbiology",
issn = "0266-8254",
publisher = "Wiley-Blackwell Publishing Ltd",
number = "8",

}

Download

TY - JOUR

T1 - Assessment of oxygen kinetic parameters for closely related ammonia-oxidizing bacteria

AU - Saha, Pallabita

AU - Kniggendorf, Ann Kathrin

AU - Pommerening-Röser, Andreas

AU - Nogueira, Regina

N1 - Publisher Copyright: © The Author(s) 2024. Published by Oxford University Press on behalf of Applied Microbiology International. All rights reserved.

PY - 2024/8

Y1 - 2024/8

N2 - The reaction kinetics of lithotrophic ammonia-oxidizing bacteria (AOB) are strongly dependent on dissolved oxygen (DO) as their metabolism is an aerobic process. In this study, we estimate the kinetic parameters, including the oxygen affinity constant (Km[O2]) and the maximum oxygen consumption rate (Vmax[O2]), of different AOB species, by fitting the data to the Michaelis–Menten equation using nonlinear regression analysis. An example for three different species of Nitrosomonas bacteria (N. europaea, N. eutropha, and N. mobilis) in monoculture is given, finding a Km[O2] of 0.25 ± 0.05 mg l−1, 0.47 ± 0.09 mg l−1, and 0.28 ± 0.08 mg l−1, and a Vmax[O2] of 0.07 ± 0.04 pg h−1cell−1, 0.25 ± 0.06 pg h−1cell−1, and 0.02 ± 0.001 pg h−1cell−1 for N. europaea, N. eutropha, and N. mobilis, respectively. This study shows that of the analyzed AOB, N. europaea has the highest affinity towards oxygen and N. eutropha the lowest affinity towards oxygen, indicating that the former can convert ammonia even under low DO conditions. These results improve the understanding of the ecophysiology of AOB in the environment. The accuracy of mathematically modelled ammonia oxidation can be improved, allowing the implementation of better management practices to restore the nitrogen cycle in natural and engineered water systems.

AB - The reaction kinetics of lithotrophic ammonia-oxidizing bacteria (AOB) are strongly dependent on dissolved oxygen (DO) as their metabolism is an aerobic process. In this study, we estimate the kinetic parameters, including the oxygen affinity constant (Km[O2]) and the maximum oxygen consumption rate (Vmax[O2]), of different AOB species, by fitting the data to the Michaelis–Menten equation using nonlinear regression analysis. An example for three different species of Nitrosomonas bacteria (N. europaea, N. eutropha, and N. mobilis) in monoculture is given, finding a Km[O2] of 0.25 ± 0.05 mg l−1, 0.47 ± 0.09 mg l−1, and 0.28 ± 0.08 mg l−1, and a Vmax[O2] of 0.07 ± 0.04 pg h−1cell−1, 0.25 ± 0.06 pg h−1cell−1, and 0.02 ± 0.001 pg h−1cell−1 for N. europaea, N. eutropha, and N. mobilis, respectively. This study shows that of the analyzed AOB, N. europaea has the highest affinity towards oxygen and N. eutropha the lowest affinity towards oxygen, indicating that the former can convert ammonia even under low DO conditions. These results improve the understanding of the ecophysiology of AOB in the environment. The accuracy of mathematically modelled ammonia oxidation can be improved, allowing the implementation of better management practices to restore the nitrogen cycle in natural and engineered water systems.

KW - ammonia oxidizing bacteria (aob)

KW - kinetics

KW - non-linear regression analysis

KW - oxygen affinity constant

KW - parameter estimation

UR - http://www.scopus.com/inward/record.url?scp=85202082445&partnerID=8YFLogxK

U2 - 10.1093/lambio/ovae076

DO - 10.1093/lambio/ovae076

M3 - Article

C2 - 39108081

AN - SCOPUS:85202082445

VL - 77

JO - Letters in applied microbiology

JF - Letters in applied microbiology

SN - 0266-8254

IS - 8

M1 - ovae076

ER -

Von denselben Autoren