Aptamer-modified magnetic beads in affinity separation of proteins

Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review

Autorschaft

  • Guohong Zhu
  • Johanna Gabriela Walter

Organisationseinheiten

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Details

OriginalspracheEnglisch
Seiten (von - bis)67-82
Seitenumfang16
FachzeitschriftMethods in Molecular Biology
Jahrgang1286
PublikationsstatusVeröffentlicht - 2015

Abstract

Aptamers are valuable alternative ligands for affinity separations. Here, we describe the aptamer-based affinity separation of His-tagged proteins using an aptamer directed against the His-tag. The immobilization of the aptamer to magnetic beads is described as well as the aptamer-based purification and proper methods for the characterization of the process. Moreover, indications for the transfer of the process to other aptamers are given.

ASJC Scopus Sachgebiete

  • Biochemie, Genetik und Molekularbiologie (insg.)
  • Molekularbiologie
  • Biochemie, Genetik und Molekularbiologie (insg.)
  • Genetik

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Aptamer-modified magnetic beads in affinity separation of proteins. / Zhu, Guohong; Walter, Johanna Gabriela.
in: Methods in Molecular Biology, Jahrgang 1286, 2015, S. 67-82.

Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review

Zhu, G & Walter, JG 2015, 'Aptamer-modified magnetic beads in affinity separation of proteins', Methods in Molecular Biology, Jg. 1286, S. 67-82. https://doi.org/10.1007/978-1-4939-2447-9_7
Zhu, G., & Walter, J. G. (2015). Aptamer-modified magnetic beads in affinity separation of proteins. Methods in Molecular Biology, 1286, 67-82. https://doi.org/10.1007/978-1-4939-2447-9_7
Zhu G, Walter JG. Aptamer-modified magnetic beads in affinity separation of proteins. Methods in Molecular Biology. 2015;1286:67-82. doi: 10.1007/978-1-4939-2447-9_7
Zhu, Guohong ; Walter, Johanna Gabriela. / Aptamer-modified magnetic beads in affinity separation of proteins. in: Methods in Molecular Biology. 2015 ; Jahrgang 1286. S. 67-82.
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