A spatio-temporal transcriptomic and proteomic dataset of developing Brassica napus seeds

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autorschaft

  • Dominic Knoch
  • Nils Rugen
  • Johannes Thiel
  • Marc C. Heuermann
  • Markus Kuhlmann
  • Paride Rizzo
  • Rhonda C. Meyer
  • Steffen Wagner
  • Jos H. M. Schippers
  • Hans-Peter Braun
  • Thomas Altmann

Externe Organisationen

  • Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK)
Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Aufsatznummer759
FachzeitschriftScientific data
Jahrgang12
Ausgabenummer1
PublikationsstatusVeröffentlicht - 7 Mai 2025

Abstract

Oilseed rape (Brassica napus) seeds are of major economic and nutritional value since they are rich in both oil and proteins, which accumulate predominantly in the embryonic cotyledons during the filling period. Developmental phases such as embryogenesis, seed filling, and maturation have been associated with specific changes in the transcriptional landscape and are controlled by interactions of regulatory components, particularly transcription factors and cis-regulatory elements. However, the global changes on the protein level remain largely elusive. Here, we investigated the dynamics of seed development by an integrative analysis of the seed transcriptome and proteome. Plants of the winter-type cultivar Express 617 were grown under controlled, field-like conditions in the IPK PhenoSphere, and developing seeds were collected for temporally and spatially resolved multi-omics analyses. The dataset covers five stages, from pre-storage to seed maturation, and includes spatial information on four dissected organs/tissues. It provides comprehensive insights into differentiation and developmental processes of the Brassica napus seed and may serve as starting point to select potentially important genes for detailed functional investigations.

ASJC Scopus Sachgebiete

Zitieren

A spatio-temporal transcriptomic and proteomic dataset of developing Brassica napus seeds. / Knoch, Dominic; Rugen, Nils; Thiel, Johannes et al.
in: Scientific data, Jahrgang 12, Nr. 1, 759, 07.05.2025.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Knoch, D, Rugen, N, Thiel, J, Heuermann, MC, Kuhlmann, M, Rizzo, P, Meyer, RC, Wagner, S, Schippers, JHM, Braun, H-P & Altmann, T 2025, 'A spatio-temporal transcriptomic and proteomic dataset of developing Brassica napus seeds', Scientific data, Jg. 12, Nr. 1, 759. https://doi.org/10.1038/s41597-025-05115-4
Knoch, D., Rugen, N., Thiel, J., Heuermann, M. C., Kuhlmann, M., Rizzo, P., Meyer, R. C., Wagner, S., Schippers, J. H. M., Braun, H.-P., & Altmann, T. (2025). A spatio-temporal transcriptomic and proteomic dataset of developing Brassica napus seeds. Scientific data, 12(1), Artikel 759. https://doi.org/10.1038/s41597-025-05115-4
Knoch D, Rugen N, Thiel J, Heuermann MC, Kuhlmann M, Rizzo P et al. A spatio-temporal transcriptomic and proteomic dataset of developing Brassica napus seeds. Scientific data. 2025 Mai 7;12(1):759. doi: 10.1038/s41597-025-05115-4
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abstract = "Oilseed rape (Brassica napus) seeds are of major economic and nutritional value since they are rich in both oil and proteins, which accumulate predominantly in the embryonic cotyledons during the filling period. Developmental phases such as embryogenesis, seed filling, and maturation have been associated with specific changes in the transcriptional landscape and are controlled by interactions of regulatory components, particularly transcription factors and cis-regulatory elements. However, the global changes on the protein level remain largely elusive. Here, we investigated the dynamics of seed development by an integrative analysis of the seed transcriptome and proteome. Plants of the winter-type cultivar Express 617 were grown under controlled, field-like conditions in the IPK PhenoSphere, and developing seeds were collected for temporally and spatially resolved multi-omics analyses. The dataset covers five stages, from pre-storage to seed maturation, and includes spatial information on four dissected organs/tissues. It provides comprehensive insights into differentiation and developmental processes of the Brassica napus seed and may serve as starting point to select potentially important genes for detailed functional investigations.",
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AU - Knoch, Dominic

AU - Rugen, Nils

AU - Thiel, Johannes

AU - Heuermann, Marc C.

AU - Kuhlmann, Markus

AU - Rizzo, Paride

AU - Meyer, Rhonda C.

AU - Wagner, Steffen

AU - Schippers, Jos H. M.

AU - Braun, Hans-Peter

AU - Altmann, Thomas

PY - 2025/5/7

Y1 - 2025/5/7

N2 - Oilseed rape (Brassica napus) seeds are of major economic and nutritional value since they are rich in both oil and proteins, which accumulate predominantly in the embryonic cotyledons during the filling period. Developmental phases such as embryogenesis, seed filling, and maturation have been associated with specific changes in the transcriptional landscape and are controlled by interactions of regulatory components, particularly transcription factors and cis-regulatory elements. However, the global changes on the protein level remain largely elusive. Here, we investigated the dynamics of seed development by an integrative analysis of the seed transcriptome and proteome. Plants of the winter-type cultivar Express 617 were grown under controlled, field-like conditions in the IPK PhenoSphere, and developing seeds were collected for temporally and spatially resolved multi-omics analyses. The dataset covers five stages, from pre-storage to seed maturation, and includes spatial information on four dissected organs/tissues. It provides comprehensive insights into differentiation and developmental processes of the Brassica napus seed and may serve as starting point to select potentially important genes for detailed functional investigations.

AB - Oilseed rape (Brassica napus) seeds are of major economic and nutritional value since they are rich in both oil and proteins, which accumulate predominantly in the embryonic cotyledons during the filling period. Developmental phases such as embryogenesis, seed filling, and maturation have been associated with specific changes in the transcriptional landscape and are controlled by interactions of regulatory components, particularly transcription factors and cis-regulatory elements. However, the global changes on the protein level remain largely elusive. Here, we investigated the dynamics of seed development by an integrative analysis of the seed transcriptome and proteome. Plants of the winter-type cultivar Express 617 were grown under controlled, field-like conditions in the IPK PhenoSphere, and developing seeds were collected for temporally and spatially resolved multi-omics analyses. The dataset covers five stages, from pre-storage to seed maturation, and includes spatial information on four dissected organs/tissues. It provides comprehensive insights into differentiation and developmental processes of the Brassica napus seed and may serve as starting point to select potentially important genes for detailed functional investigations.

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