A novel LED-based 2D-fluorescence spectroscopy system for in-line bioprocess monitoring of Chinese hamster ovary cell cultivations—Part II

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OriginalspracheEnglisch
Seiten (von - bis)341-351
Seitenumfang11
FachzeitschriftEngineering in life sciences
Jahrgang19
Ausgabenummer5
Frühes Online-Datum4 März 2019
PublikationsstatusVeröffentlicht - 6 Mai 2019

Abstract

This study was performed in order to evaluate a new LED-based 2D-fluorescence spectrometer for in-line bioprocess monitoring of Chinese hamster ovary (CHO) cell culture processes. The new spectrometer used selected excitation wavelengths of 280, 365, and 455 nm to collect spectral data from six 10-L fed-batch processes. The technique provides data on various fluorescent compounds from the cultivation medium as well as from cell metabolism. In addition, scattered light offers information about the cultivation status. Multivariate data analysis tools were applied to analyze the large data sets of the collected fluorescence spectra. First, principal component analysis was used to accomplish an overview of all spectral data from all six CHO cultivations. Partial least square regression models were developed to correlate 2D-fluorescence spectral data with selected critical process variables as offline reference values. A separate independent fed-batch process was used for model validation and prediction. An almost continuous in-line bioprocess monitoring was realized because 2D-fluorescence spectra were collected every 10 min during the whole cultivation. The new 2D-fluorescence device demonstrates the significant potential for accurate prediction of the total cell count, viable cell count, and the cell viability. The results strongly indicated that the technique is particularly capable to distinguish between different cell statuses inside the bioreactor. In addition, spectral data provided information about the lactate metabolism shift and cellular respiration during the cultivation process. Overall, the 2D-fluorescence device is a highly sensitive tool for process analytical technology applications in mammalian cell cultures.

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A novel LED-based 2D-fluorescence spectroscopy system for in-line bioprocess monitoring of Chinese hamster ovary cell cultivations—Part II. / Claßen, Jens; Graf, Alexander; Aupert, Florian et al.
in: Engineering in life sciences, Jahrgang 19, Nr. 5, 06.05.2019, S. 341-351.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

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title = "A novel LED-based 2D-fluorescence spectroscopy system for in-line bioprocess monitoring of Chinese hamster ovary cell cultivations—Part II",
abstract = "This study was performed in order to evaluate a new LED-based 2D-fluorescence spectrometer for in-line bioprocess monitoring of Chinese hamster ovary (CHO) cell culture processes. The new spectrometer used selected excitation wavelengths of 280, 365, and 455 nm to collect spectral data from six 10-L fed-batch processes. The technique provides data on various fluorescent compounds from the cultivation medium as well as from cell metabolism. In addition, scattered light offers information about the cultivation status. Multivariate data analysis tools were applied to analyze the large data sets of the collected fluorescence spectra. First, principal component analysis was used to accomplish an overview of all spectral data from all six CHO cultivations. Partial least square regression models were developed to correlate 2D-fluorescence spectral data with selected critical process variables as offline reference values. A separate independent fed-batch process was used for model validation and prediction. An almost continuous in-line bioprocess monitoring was realized because 2D-fluorescence spectra were collected every 10 min during the whole cultivation. The new 2D-fluorescence device demonstrates the significant potential for accurate prediction of the total cell count, viable cell count, and the cell viability. The results strongly indicated that the technique is particularly capable to distinguish between different cell statuses inside the bioreactor. In addition, spectral data provided information about the lactate metabolism shift and cellular respiration during the cultivation process. Overall, the 2D-fluorescence device is a highly sensitive tool for process analytical technology applications in mammalian cell cultures.",
keywords = "2D-fluorescence spectroscopy, bioprocess monitoring, CHO cell cultivation, multivariate data analysis, PAT",
author = "Jens Cla{\ss}en and Alexander Graf and Florian Aupert and D{\"o}rte Solle and Marek H{\"o}hse and Thomas Scheper",
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TY - JOUR

T1 - A novel LED-based 2D-fluorescence spectroscopy system for in-line bioprocess monitoring of Chinese hamster ovary cell cultivations—Part II

AU - Claßen, Jens

AU - Graf, Alexander

AU - Aupert, Florian

AU - Solle, Dörte

AU - Höhse, Marek

AU - Scheper, Thomas

N1 - Funding information: The authors would like to thank the federal ministry of education and research for financial support.

PY - 2019/5/6

Y1 - 2019/5/6

N2 - This study was performed in order to evaluate a new LED-based 2D-fluorescence spectrometer for in-line bioprocess monitoring of Chinese hamster ovary (CHO) cell culture processes. The new spectrometer used selected excitation wavelengths of 280, 365, and 455 nm to collect spectral data from six 10-L fed-batch processes. The technique provides data on various fluorescent compounds from the cultivation medium as well as from cell metabolism. In addition, scattered light offers information about the cultivation status. Multivariate data analysis tools were applied to analyze the large data sets of the collected fluorescence spectra. First, principal component analysis was used to accomplish an overview of all spectral data from all six CHO cultivations. Partial least square regression models were developed to correlate 2D-fluorescence spectral data with selected critical process variables as offline reference values. A separate independent fed-batch process was used for model validation and prediction. An almost continuous in-line bioprocess monitoring was realized because 2D-fluorescence spectra were collected every 10 min during the whole cultivation. The new 2D-fluorescence device demonstrates the significant potential for accurate prediction of the total cell count, viable cell count, and the cell viability. The results strongly indicated that the technique is particularly capable to distinguish between different cell statuses inside the bioreactor. In addition, spectral data provided information about the lactate metabolism shift and cellular respiration during the cultivation process. Overall, the 2D-fluorescence device is a highly sensitive tool for process analytical technology applications in mammalian cell cultures.

AB - This study was performed in order to evaluate a new LED-based 2D-fluorescence spectrometer for in-line bioprocess monitoring of Chinese hamster ovary (CHO) cell culture processes. The new spectrometer used selected excitation wavelengths of 280, 365, and 455 nm to collect spectral data from six 10-L fed-batch processes. The technique provides data on various fluorescent compounds from the cultivation medium as well as from cell metabolism. In addition, scattered light offers information about the cultivation status. Multivariate data analysis tools were applied to analyze the large data sets of the collected fluorescence spectra. First, principal component analysis was used to accomplish an overview of all spectral data from all six CHO cultivations. Partial least square regression models were developed to correlate 2D-fluorescence spectral data with selected critical process variables as offline reference values. A separate independent fed-batch process was used for model validation and prediction. An almost continuous in-line bioprocess monitoring was realized because 2D-fluorescence spectra were collected every 10 min during the whole cultivation. The new 2D-fluorescence device demonstrates the significant potential for accurate prediction of the total cell count, viable cell count, and the cell viability. The results strongly indicated that the technique is particularly capable to distinguish between different cell statuses inside the bioreactor. In addition, spectral data provided information about the lactate metabolism shift and cellular respiration during the cultivation process. Overall, the 2D-fluorescence device is a highly sensitive tool for process analytical technology applications in mammalian cell cultures.

KW - 2D-fluorescence spectroscopy

KW - bioprocess monitoring

KW - CHO cell cultivation

KW - multivariate data analysis

KW - PAT

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DO - 10.1002/elsc.201800146

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JF - Engineering in life sciences

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ER -

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